Axioplan 2 upright light microscope

Manufactured by Zeiss

Sourced in Germany

The Zeiss Axioplan 2 Upright Light Microscope is a high-performance optical microscope designed for advanced research and imaging applications. It features a sturdy, ergonomic design and a wide range of optical components to accommodate various imaging techniques, such as brightfield, darkfield, and phase contrast microscopy.

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Lab products found in correlation

Axiocam 305 color, by Zeiss (2 mentions) Axiocam mrm, by Zeiss (1 mentions) Axiovision version 4, by Zeiss (1 mentions) Plan apochromat, by Zeiss (1 mentions) Progres capturepro 2, by Jenoptik (1 mentions) Progres c3 digital camera, by Jenoptik (1 mentions)

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Axioplan 2 microscope (979 citations) Axioplan 2 (859 citations) Axioplan microscope (451 citations) Axioplan light microscope (66 citations) Upright microscope (42 citations) Axioplan 2 light microscope (27 citations) Axioplan upright microscope (10 citations) Axioplan 2 Upright (7 citations) Upright light microscope (6 citations) Axioplan-2 light (2 citations)

5 protocols using axioplan 2 upright light microscope

B. subtilis Microscopy Protocol

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B. subtilis cells in exponential phase (OD₆₀₀ 0.1–0.3) were treated with the indicated concentrations of compounds or left untreated (control). At 30 min, aliquots were retrieved, centrifuged (16,000 rcf for 3 min) resuspended in PBS. This bacterial suspension was pipetted onto 2% (w/v) agarose pads for phase-contrast microscopy using the Zeiss Axioplan 2 Upright Light Microscope.
For fluorescence microscopy, bacterial cells in exponential phase (OD₆₀₀ 0.1–0.3) were treated with indicated concentrations of compounds and incubated for 30 min. For the final 10 min of incubation, AM466 was added to a final concentration of 25 µg/mL. At 30 min, aliquots were retrieved and subjected to the centrifugation and resuspension protocol as described above. DAPI (final concentration of 200 ng/mL) was subsequently added to bacterial suspensions. Following a further 5-min incubation, these suspensions were pipetted onto 2% agarose pads for fluorescence microscopy using the Zeiss Axioplan 2 Upright Light Microscope at 100 × magnification using a Plan ApoChromat (100 × NA 1.4; Zeiss) phase-contrast objective and an AxioCam MRm cooled charge-coupled-device (CCD) camera. All images were analysed using Zeiss AxioVision version 4.8.

Galletta M., Reekie T.A., Nagalingam G., Bottomley A.L., Harry E.J., Kassiou M, & Triccas J.A. (2020). Rapid Antibacterial Activity of Cannabichromenic Acid against Methicillin-Resistant Staphylococcus aureus. Antibiotics, 9(8), 523.

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Quantifying Leaf Trichome Density

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The upper parts of second leaves (same as those used for applying clip cages) were collected, and chlorophyll was bleached by 70% ethanol at 85 °C for 8 min, then rinsed with water. The tissue was placed on glass microscope slides facing to the adaxial side (leaf surface up). Trichome density images were photographed with a digital camera (Axiocam 305 color) connected to a Zeiss Axioplan 2 Upright Light Microscope (Zeiss, Oberkochen, Germany). For each leaf, nine photos were taken, including three from each side (left, right), and three from the middle (top, medium, and bottom positions). We counted the number of trichomes on the edges per mm and the density in mm² using ImageJ software (https://imagej.nih.gov/ij/).

Batyrshina Z.S., Yaakov B., Shavit R., Singh A, & Tzin V. (2020). Comparative transcriptomic and metabolic analysis of wild and domesticated wheat genotypes reveals differences in chemical and physical defense responses against aphids. BMC Plant Biology, 20, 19.

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Trichome Density Quantification in Leaf Samples

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The middle sections of leaf-1, leaf-2, and leaf-3, from untreated and aphid-infested plants, were collected. From each leaf, 1 cm² was excised, cleared with 80% ethanol at 85°C for 15 min, and rinsed with distilled water. The tissue segment was placed on glass microscope slides facing the adaxial side, and the total non-glandular trichomes were counted (Batyrshina et al., 2020b (link)). Images were acquired with a digital camera (Axiocam 305 color) connected to a Zeiss Axioplan 2 Upright Light Microscope (Zeiss, Oberkochen, Germany). For each accession, three images per plant were captured from the middle portion of the leaves, and trichome density in mm² was calculated using ImageJ software (see text footnote 1).

Singh A., Dilkes B., Sela H, & Tzin V. (2021). The Effectiveness of Physical and Chemical Defense Responses of Wild Emmer Wheat Against Aphids Depends on Leaf Position and Genotype. Frontiers in Plant Science, 12, 667820.

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Histological Analysis of Vertebral Columns

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Fixed and decalcified vertebral column segments were dehydrated in a graded series of alcohol before being paraffin‐embedded in the coronal plane. The embedded segments were serially sectioned into 10 μm thick slices and mounted onto glass slides. Individual paraffin sections were deparaffinized by running them through xylene, alcohol, and water. Hematoxylin‐eosin and bone and cartilage staining techniques were carried out according to the standard protocols described in detail in Romeis Mikroskopische Technik (19th edition) (Mulisch & Welsch, 2015 ). Alcian blue was used to stain cartilage and Light green was used to stain bone. Goldner's Masson's and Azan trichrome stains were used to stain both bone and cartilage. Elastica van Gieson and Weigert's Resorcin Fuchsin stain were used to stain collagen and elastin, respectively. After staining, the slides were rinsed, dehydrated in alcohol, cleared in xylene, and covered with a coverslip. The slides were then evaluated under an Axioplan 2 upright light microscope (Zeiss), photographed with a ProgRes C3 digital camera (Jenoptik), and documented with a digital recording system (ProgRes CapturePro 2.8.8; Jenoptik).

Brendler J., Winter K., Lochhead P., Schulz A, & Ricken A.M. (2021). Histological differences between lumbar and tail intervertebral discs in mice. Journal of Anatomy, 240(1), 84-93.

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Trichome Density Quantification in Tef Leaves

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Tef plants from the three accessions were grown for 1 month (no aphids were applied on these leaves). Then, 2 cm sections were sampled from the widest part of three leaves: (i) lower leaf (a first leaf from the base), (ii) middle leaf, and (iii) upper leaf. The three leaves were dissected, bleached in 80% (v/v) ethanol, boiled at 90°C for 20 min, and washed with distilled water as previously described (Batyrshina et al., 2020b (link)). For trichome visualization, leaves were mounted on microscope slides with the adaxial side facing up, covered with glass coverslips. A digital camera connected to an Axioplan 2 Upright Light Microscope (Zeiss, Oberkochen, Germany) was used for imaging. For each tef accession, five biological replicates with two pictures per leaf were taken. For density quantification, trichomes were counted using ImageJ software¹ and normalized per mm².

Gyan N.M., Yaakov B., Weinblum N., Singh A., Cna’ani A., Ben-Zeev S., Saranga Y, & Tzin V. (2020). Variation Between Three Eragrostis tef Accessions in Defense Responses to Rhopalosiphum padi Aphid Infestation. Frontiers in Plant Science, 11, 598483.

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