B16.F10 murine metastatic melanoma, A375 human melanoma, MB-49 murine and human T-24 bladder tumor cell lines, TC-1 murine and A-549 NSCLC lung tumor cell lines, O627 murine glioblastoma cell lines, Hepa1-6 murine hepatocarcinoma cell lines, CHO hamster ovary cell lines and SV-40-induced IC-21 murine macrophage cell lines were obtained from ATCC (Manassas, VA, USA). The RG-1 human glioblastoma cell line was a gift of J. L. Fernandez-Luna (HUMV, Santander, Spain). C57BL/6 male or female mice (Charles River, L’Abresle, France) 8–12 months of age were used in the study.
A375 human melanoma
Manufactured by American Type Culture Collection
Sourced in United States
The A375 human melanoma cell line is a well-characterized in vitro model derived from a malignant melanoma. It is a widely used cell line for cancer research and drug development.
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Lab products found in correlation
B16f10 mouse melanoma, by American Type Culture Collection (2 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
A2780 human ovarian cancer cells, by Merck Group (1 mentions)
N1250, by Solarbio (1 mentions)
Forma 3111, by Thermo Fisher Scientific (1 mentions)
P1400, by Solarbio (1 mentions)
A549 human lung adenocarcinoma, by American Type Culture Collection (1 mentions)
Penicillin strep, by Merck Group (1 mentions)
Mda mb 231 human breast adenocarcinoma, by American Type Culture Collection (1 mentions)
5 protocols using a375 human melanoma
1
Diverse Tumor Cell Lines for Research
2
Cell Culture and IFN-γ Stimulation
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The EL4 mouse T cells were purchased from CTCC (China Centre for Type Culture Collection, CTCC). The CAL27 human oral cancer, MDA-MB-231 human breast cancer, H1264 human lung cancer, MC38 mouse colon cancer, A375 human melanoma and B16F10 mouse melanoma cells were purchased from ATCC. CAL27, MDA-MB-231, MC38 and A375 were cultured in DMEM (Sigma) supplemented with 10% fetal bovine serum (FBS) (Invitrogen). EL4 and H1264 were cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% FBS. All cells were cultured at 37 °C in a 5% CO2 incubator. For stimulation with IFN-γ, cells were incubated with 10 ng ml−1 of recombinant human IFN-γ (Sino Biological Inc.) for 48 h.
3
Cell Line Cultivation and Characterization
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The cell lines used in this study included MCF-7 human mammary cancer cells (ATCC, HTB-22), A2780 human ovarian cancer cells (Sigma, 93112519), PaTu-8902 pancreatic adenocarcinoma cells (DSMZ, n° ACC179), HuH-7 human hepatocarcinoma cells (JCRB Cell Bank JCRB043, National Institute of Biomedical Innovation), MCF-10A normal breast cell (ATCC, CRL-10317), DU145 prostatic adenocarcinoma (ATCC, HTB-81), A375 human melanoma cells (ATCC, CRL 1619) and MDA-MB-231 human mammary cancer cells (ATCC, HTB-26). All cells were grown in Dulbecco’s modified Eagle’s medium supplemented with 10% foetal bovine serum. The cells were incubated at 37 °C in a humidified atmosphere with 5% CO2 .
4
Cultivation of A375 and B16-F10 Melanoma Cells
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The A375 human melanoma and B16-F10 mouse melanoma cell lines were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA), while B16-F10-luciferase (B16-F10-luc) cells were purchased from Shanghai Model Organisms Center (Shanghai, China). The A375 and B16-F10-luc cells were cultured in Dulbecco's modified Eagle's medium-high glucose (SH30243.01, HyClone, Logan, UT, USA) containing 10% fetal bovine serum (FBS; SH30084.03, HyClone), 1% penicillin/1% streptomycin (P1400, Solarbio, Beijing, China), 1% L-glutamine (G0200, Solarbio), and 1% nonessential amino acid (N1250, Solarbio). The cells were cultured in an incubator at 37°C with 5% CO2 (Forma 3111, Thermo Fisher Scientific, Waltham, MA, USA).
5
Cell Culture Conditions for Cancer Cell Lines
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A375 human melanoma, A549 human lung adenocarcinoma, and MDA-MB-231 human breast adenocarcinoma cell lines were purchased from the American Type Culture Collection (ATCC); HaCaT-human immortalized keratinocyte cell line was provided by the University of Debrecen, Hungary as a kind gift.
All the cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA) high-glucose medium supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin/Strep, 10,000 IU/ml (Sigma-Aldrich; Merck KGaA). The cells were incubated under standard temperature conditions of 37°C and humidity containing 5% CO2.
All the cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA) high-glucose medium supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin/Strep, 10,000 IU/ml (Sigma-Aldrich; Merck KGaA). The cells were incubated under standard temperature conditions of 37°C and humidity containing 5% CO2.
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