Total C and N of fine‐roots were determined using an Elementar CHNS analyzer (Vario EL III, Elementar Analyser Systeme; GmbH, Germany); whereas total P and K were analyzed with the HNO3 digestion method using a 6300 ICP‐AES (Thermo Scientific, USA). Total N of soil was measured with the semimicro‐kjeldahl method using a kjeldahl apparatus (Kjeldahl 2200 Auto Distillation Unit; FOSS, Sweden); whereas total P and K concentrations were obtained with the HF‐HClO4‐HNO3 digestion method using a 6300 ICP‐AES (Thermo Scientific, USA).
Icp aes 6300
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ICP-AES (6300) is an analytical instrument used for the detection and quantification of multiple elements in a sample. It utilizes inductively coupled plasma to atomize and ionize the sample, and atomic emission spectroscopy to measure the characteristic wavelengths emitted by the excited atoms.
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5 protocols using icp aes 6300
1
Elemental Analysis of Fine-Roots and Soil
2
Evaluating Heavy Metal Uptake in Plants
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After the pot experiment, the plants were collected and the roots were soaked with 0.01 M EDTA for 30 min to remove HMs adhering to root surface. Deionised water was used to wash the plant samples three times and the fresh weight of roots, stems and leaves of plants were measured, respectively. Then, the plant samples were oven dried for 2 h at 105 °C and then dried at 75 °C to get a constant weight. After being digested with HNO3-HClO4 (4:1), the concentration of multiple metals in plants was determined by ICP-AES (6300; Thermo Fisher, Waltham, MA, USA).
3
Evaluating Mineralizing Film Ion Release
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The mineralizing film (15 × 40 × 0.1 mm3) was immersed in 20 mL of NaCl solution (133 mmol/L) buffered with 50 mmol/L HEPES (pH = 7.0, 37 °C).35 (link) The concentrations of Ca, P, and F ions released from the film were measured at 0, 2, 4, 8, 12 and 24 h. At each time interval, an aliquot (0.5 mL) of immersion solution was retrieved and replenished with fresh solution. The concentrations of Ca and P ions were measured via inductively coupled plasma atomic emission spectrometry (ICP-AES 6300, Thermo Fisher).21 (link) Moreover, the amount of F ions released was measured by a fluoride ion selective electrode (Shanghai D&B Biological Science and Technology Co., Ltd., Shanghai China). The solutions collected as aforementioned were first diluted to a concentration within the ranges of measurement and then mixed with ionic strength adjustment buffer (ISAB) (containing 0.2 M acetic acid, 1.0 M sodium acetate, 1.0 M NaCl and 0.02 M CDTA) in a volume ratio of 50:1. Fluoride standard solutions ranging from 1 × 10−6 to 1 × 10−1 mol/L were measured to form a calibration curve, which was used to calculate the F ions concentration.36 (link) All measurements were performed in triplicate, and the averaged values were used.
4
Comprehensive Chemical Characterization of Substrate
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The pH value was determined with a Delta 320 pH meter (Mettler-Toledo International Inc., Switzerland) at substrate: water = 1:5. The content of organic matter was determined using the potassium dichromate volumetric method. Using the Kjeldahl determination, the content of total nitrogen was investigated in substrate after heating digestion by concentrated sulfuric acid. The contents of total phosphorus, total potassium, and heavy metals were determined by ICP-AES (6,300, Thermo Fisher Scientific Inc., USA) after microwave digestion.
5
Nutrient Analysis of Suaeda psammophila Sand Barrier
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The nutrient contents C, N, P, and K of the S. psammophila sand barrier were determined after the samples were crushed by a grinder and ground into powder through a 40-mesh to 60-mesh sieve. Soil samples collected from the field were naturally air-dried, and their nutrient content was determined. The total C contents of the S. psammophila sand barrier and the surrounding soil were determined via the potassium dichromate volumetric method. The N content was determined via the perchloric acid-sulfuric acid digestion method. The total N content of the soil was measured with the semimicrokjeldahl method using a Kjeldahl apparatus (Kjeldahl 2200 Auto Distillation Unit, FOSS, Scandinavia, Sweden), whereas the total P and K concentrations were obtained via the HF-HClO4-HNO3 digestion method using a 6300 ICP-AES (Thermo Scientific, Waltham, MA, USA).
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