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2 protocols using heptadecafluorooctanesulfonic acid potassium salt

1

Synthesis and Characterization of PFBS and PFOS

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Potassium nonafluoro-1-butanesulfonate (K+PFBS, CAS No.: 29420–49-3, ≥ 98% purity) and Heptadecafluorooctanesulfonic acid potassium salt (K+PFOS, CAS No.:2795–39-3, ≥ 98% purity) were purchased from Sigma-Aldrich (St. Louis, MO). PFBS and PFOS stock solutions were prepared by dissolving the compounds in ultrapure distilled water at 100 and 10 μM or 100 and 10 mM, respectively. Forskolin (Wako chemicals, Japan) stock solution was reconstituted in DMSO at 80 mM.
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2

Quantification of PFOS and PFOA

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Analytical grade heptadecafluorooctanesulfonic acid potassium salt (PFOS, 98%), perfluorooctanoic acid (PFOA, 96%), HPLC grade methanol (>99.8%), potassium persulfate (>99%), sodium periodate (>99.8%), sodium iodate (>99%), sodium perchlorate (>98%), sodium chlorate (>99.0%), acetonitrile (>99.9%) and HPLC water were purchased from Sigma Aldrich, USA. Stable-isotope surrogate of sodium perfluorooctanesulfonate (13C8 PFOS, 99%) and perfluorooctanoic acid (13C8 PFOA, 99%) were procured from Cambridge isotope laboratories, Inc. Oasis weak anion exchange (WAX; 3 cc cartridge 60 mg 30 μm) cartridges were purchased from Water Corp (Milford, MA, USA) for solid-phase extraction (SPE) of PFOS and PFOA. Argon was purchased from Airgas., Philadelphia, USA. The aqueous solutions were prepared with Milli-Q water (>18.2 MΩ cm−1 resistivity). All materials were used as received.
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