To each membrane support (12-mm Transwell®, Corning Costar, United States), 300 μl TRIzolTM reagent (Cat. 15596081, Invitrogen, United States) was added to lyse the cells. Total RNA was then extracted using the RNA extraction kit (Cat. E1011-A, ZYMO Research, United States). About 500 ng total RNA was reverse transcribed to cDNA with the oligo (dT)20 primer (Cat. 18418020, Invitrogen, United States) or random hexamers (Cat. N8080127, Invitrogen, United States) using the SuperScript™ IV First-Strand Synthesis System (Cat. 18091050, Invitrogen, United States), following the manufacturer’s instruction.
12 mm transwell
Manufactured by Corning
Sourced in United States
The 12-mm Transwell is a cell culture insert designed for a range of in vitro applications. It features a 12-millimeter diameter and a semi-permeable membrane that allows for the study of cell migration, invasion, and transport processes. The product specifications and technical details are available upon request.
Automatically generated - may contain errors
2 protocols using 12 mm transwell
1
Total RNA Extraction and cDNA Synthesis
2
Total RNA Extraction and Reverse Transcription
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To each membrane support (12-mm Transwell®, Corning Costar, United States), 300 μl TriZOL® reagent (Cat. 15596081, Invitrogen, United States) were added to lyse the cells. Total RNA was then extracted with RNA extraction kit (Cat. E1011-A, ZYMO Research, United States). About 500 ng total RNA were reverse transcribed to cDNA with oligo(dT)20 primer (Cat. 18418020, Invitrogen, United States) or random hexamers (Cat. N8080127, Invitrogen, United States) using SuperScriptTM IV First-Strand Synthesis System (Cat. 18091050, Invitrogen, United States) following the manufacturer’s instruction.
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