αcleaved caspase 8

Manufactured by Cell Signaling Technology

Sourced in United States

The αcleaved caspase-8 antibody is a laboratory research tool used to detect the cleaved form of caspase-8 protein. Caspase-8 is a protease enzyme involved in the initiation of apoptosis, or programmed cell death. The αcleaved caspase-8 antibody specifically recognizes the cleaved, active form of caspase-8, which is a key step in the apoptotic signaling pathway.

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Lab products found in correlation

α β actin, by Merck Group (2 mentions) α flag, by Merck Group (1 mentions) 13c4 fumarate, by Cambridge Isotopes (1 mentions) α tubulin, by Merck Group (1 mentions) α cleaved caspase 3, by Abcam (1 mentions) 13c3 pyruvate, by Cambridge Isotopes (1 mentions) α5hmc, by Active Motif (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) α ripk3, by Novus Biologicals (1 mentions) α rabbit hrp, by GE Healthcare (1 mentions) α mouse hrp, by GE Healthcare (1 mentions) α cleaved caspase 3, by Cell Signaling Technology (1 mentions) P jnk, by Cell Signaling Technology (1 mentions) Protein extraction reagent, by Thermo Fisher Scientific (1 mentions) Nupage bis tris gel, by Thermo Fisher Scientific (1 mentions) α adipoq, by GeneTex (1 mentions) Immobilon p transfer membrane, by Merck Group (1 mentions) α caspase 8, by Cell Signaling Technology (1 mentions) α hsp90, by Cell Signaling Technology (1 mentions) Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)

3 protocols using αcleaved caspase 8

Metabolic Profiling and Epigenetic Analysis

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The antibodies used were αCS (SAB2701077), αACO2 (HPA001097), αOGDH (HPA020347), αSUCLG2 (HPA046705), αSDHA (HPA041981), αFH (HPA025770), αMDH2 (HPA019716), αTubulin (T8328), αKAT8 (SAB1306205), αβ-actin (A5441), and αFLAG (F3165) from Sigma; αIDH3A (ab228596), αSDHC (ab191362), αLamin B1 (ab133741), αH3K4me3 (ab8580), αH3K9me3 (ab8898), αH3K9ac (ab4441), αH3K27ac (ab4729), αpan-H3ac (ab47915), αH4K16ac (ab109463), αpan-H4ac (ab240201), αH3 (ab195277), αH4 (ab10158), αcleaved caspase-3 (ab32042), αFADD (ab108601), αKAT2A (ab217876), and αRUNX3 (ab224641) from Abcam; αcleaved caspase-8 (#9496) and αcleaved PARP (#5625) from Cell Signaling Technology; αPOLG (sc-390634) from Santa Cruz; α5hmC (#39069) from Active Motif; αZNF516 (A303-392A) from Bethyl; αMRPL39 (28165-1-AP) from Proteintech. Recombinant human KAT8 protein (SRP5220) was from Sigma and recombinant human KAT2A protein (ab198084) was from Abcam. Protease inhibitor cocktail was from Roche Applied Science. ¹³C₃-pyruvate, ¹³C₆-citrate, ¹³C₄-fumarate and ¹³C₄-malate were from Cambridge Isotope Laboratories.

Liu X., Si W., He L., Yang J., Peng Y., Ren J., Liu X., Jin T., Yu H., Zhang Z., Cheng X., Zhang W., Xia L., Huang Y., Wang Y., Liu S., Shan L., Zhang Y., Yang X., Li H., Liang J., Sun L, & Shang Y. (2021). The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry. Signal Transduction and Targeted Therapy, 6, 375.

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Liver Protein Expression Analysis

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Protein lysates were isolated from liver samples as described previously [9 (link)]. Proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to PVDF membrane and analyzed by immunoblot with the following antibodies: α-NEMO, α-β-actin (Sigma Aldrich, St. Louis, MO, USA), α-cleaved Caspase-3, α-cleaved Caspase-8, α-IKKβ, p-JNK, JNK1 (Cell Signaling Technology, Danvers, MA, USA), α-IKKα, α-RIPK3 (Novus Biologicals, Centennials, CO, USA), α-PCNA (Thermo Fisher Scientific, Waltham, MA, USA), and α-GAPDH (Bio-Rad Laborories, Hercules, CA, USA). As secondary antibodies, α-rabbit-HRP, and α-mouse-HRP (GE Healthcare, Little Chalfont, UK) were used. Densitometry was performed by using ImageJ software (ImageJ, NIH, Bethesda, MD, USA).

Koppe C., Reisinger F., Wehr K., Vucur M., Trautwein C., Tacke F., Heikenwalder M, & Luedde T. (2019). An NF-kappaB- and IKK-Independent Function of NEMO Prevents Hepatocarcinogenesis by Suppressing Compensatory Liver Regeneration. Cancers, 11(7), 999.

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Immunoblotting Protein Expression Analysis

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Tissue and whole cell lysates were prepared in Protein Extraction Reagent (Thermo Fisher) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher). Immunoblotting was performed with lysates run on 4–12% Bis-Tris NuPage gels (Life Technologies) and transferred onto Immobilon-P Transfer Membranes (Millipore) followed by antibody incubation. Immunoreactive bands were visualized by chemiluminescence. The following antibodies were used for immunoblotting: α-HSP90 (Cell Signaling #4877), α-UBE2I (Cell Signaling #4786), α-ADIPOQ (Genetex #GTX112777), α-PPARγ (Cell Signaling #2443), α-Caspase-8 (Cell Signaling #4790), and α-Cleaved Caspase-8 (Cell Signaling #8592).

Cox A.R., Chernis N., Kim K.H., Masschelin P.M., Saha P.K., Briley S.M., Sharp R., Li X., Felix J.B., Sun Z., Moore D.D., Pangas S.A, & Hartig S.M. (2021). Ube2i deletion in adipocytes causes lipoatrophy in mice. Molecular Metabolism, 48, 101221.

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