HP was purchased from Selleck (S5453, Houston, TX, USA) and dimethyl sulfoxide (DMSO, D4540) and thiazolyl blue tetrazolium bromide (MTT, M5655) were purchased from Sigma (St. Louis, MO, USA). Mouse monoclonal antibodies β-actin (BS6007M) was purchased from Bioworld Technology (Bloomington, MN, USA), and rabbit anti-AKT (9272), rabbit anti-PI3K (4249), and rabbit anti-phosphorylated AKT (P-AKT, 4060) polyclonal antibody from Cell Signaling Technology (Danvers, MA, USA). Mouse anti-BMP-7 (sc-53917) was purchased from Santa Cruz (Dallas, TX, USA) and rabbit anti-Cyclin D1 (ab226977) polyclonal antibody and rabbit anti-c-Myc (ab9106) polyclonal antibody from Abcam (Cambridge, UK).
β actin bs6007m
Manufactured by Bioworld Technology
Sourced in United States
β-actin (BS6007M) is a laboratory equipment product. It is a cytoskeletal protein that plays a crucial role in various cellular processes. This product can be used for research and experimental purposes.
Automatically generated - may contain errors
2 protocols using β actin bs6007m
1
Investigating PI3K/AKT Signaling Pathway
2
Bone Tissue Protein Immunoblotting
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Proteins were extracted from bone tissue, and immunoblotting was carried out as we described previously 31 (link). Immunoblotting was performed with the primary antibodies against Shh (ab135240; Abcam, Cambridge, MA, USA), Bmi1 (10832-1-AP; Proteintech, USA), Gli1 (ab49314; Abcam, Cambridge, MA, USA), Gli2 (18989-1-AP; Proteintech, USA), Gli3 (ab69838; Abcam, Cambridge, MA, USA), Ihh (ab39634; Abcam, Cambridge, MA, USA), Ptch1 (ab53715; Abcam, Cambridge, MA, USA), Sufu (#C54G2; Cell Signaling Technology, Beverly, MA, USA) and Smo (ab236465,Abcam, Cambridge, MA, USA). The β‐actin (BS6007M; Bioworld Technology, Bloomington, MN, USA) was used as the control for total protein.
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