Fetal calf serum fcs

Manufactured by BioConcept

Fetal calf serum (FCS) is a widely used cell culture supplement. It is obtained from the blood of bovine fetuses and provides a complex mixture of proteins, growth factors, and other nutrients essential for the growth and proliferation of cells in vitro.

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FCS (25 citations)

5 protocols using fetal calf serum fcs

Synthesis and Characterization of Amphiphilic Polymers

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Ethanol (≥99.9%, #1117272500), acetone
(≥99.9%, #650501), trypsin–EDTA solution (#T3924), bovine
serum albumin (#A2153), Rose Bengal (#330000), and Atto633 (#18620)
were purchased from Merck. Fetal calf serum (FCS, #2-01F10-I), penicillin–streptomycin
solution (Pen-Strep, #4-01F00-H), RPMI-1640 with stable glutamine
(#1–41F50-I), and Dulbecco’s phosphate-buffered saline
(PBS) without Ca²⁺/Mg²⁺ (#3-05F29-I) were purchased
from BioConcept. Dulbecco’s Modified Eagle Medium (DMEM) high
glucose (1×) with GlutaMAX (#61965-026) was purchased from Gibco.
The ROS detection assay kit was purchased from Abcam (#ab287839).
Calcein was purchased from ThermoFisher (#C481). All other chemicals
were purchased from Merck at the highest available grade and used
as received, unless stated otherwise. Hydroxyl-functionalized PDMS₂₅-b-PMOXA₁₀-OH (M_n = 2850 g mol^–1) was synthesized and
purified as previously described.^{36 (link)} Terminally
azide-functionalized PDMS-b-PMOXA was synthesized
following the method described by Meyer et al.^{37 (link)}¹H NMR (Figure S1) revealed the polymer block length to be PDMS₂₇-b-PMOXA₇-PEG₃-N₃ (M_n = 3000 g mol^–1).

Skowicki M., Hürlimann D., Tarvirdipour S., Kyropoulou M., Schoenenberger C.A., Gerber-Lemaire S, & Palivan C.G. (2024). FAP Targeting of Photosensitizer-Loaded Polymersomes for Increased Light-Activated Cell Killing. Biomacromolecules, 25(2), 754-766.

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Melanoma Cell Lines Cultivation Protocol

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Melanoma cell lines A375P, UACC62 and SK-Mel28 were obtained from ATCC and cultured in DMEM (Life technology) containing 4.5 g/l glucose supplemented with 2 mM L-glutamine and 10% Fetal Calf Serum (FCS) (BioConcept). All patient-derived melanoma cells were obtained from the University Hospital of Zurich where tumor material was obtained after surgical removal of melanomas from patients after written informed consent (19 (link)). Patient-derived melanoma cells were cultured in RPMI-1640 supplemented with GlutaMAX™ (Life technology) and 10% FCS. All cell lines were regularly tested for mycoplasma contamination with a PCR-based assay (#A8994; AppliChem).

Aloia A., Müllhaupt D., Chabbert C.D., Eberhart T., Flückiger-Mangual S., Vukolic A., Eichhoff O., Irmisch A., Alexander L.T., Scibona E., Frederick D.T., Miao B., Tian T., Cheng C., Kwong L.N., Wei Z., Sullivan R.J., Boland G.M., Herlyn M., Flaherty K.T., Zamboni N., Dummer R., Zhang G., Levesque M.P., Krek W, & Kovacs W.J. (2019). A fatty acid oxidation-dependent metabolic shift regulates the adaptation of BRAF-mutated melanoma to MAPK inhibitors. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(22), 6852-6867.

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Fluorescent DNA Labeling and Cytotoxicity Assay

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Solvents and reagents were purchased from Sigma-Aldrich unless otherwise specified. Triisopropylsilane, piperidine and N,N 0 -diisopropylcarbodiimide (DIC) were of synthesis grade. Rink Amide AM resin (0.71 mmol g À1 ) and Fmoc-Trp(Boc)-OH were purchased from IRIS Biotech GmbH. All other Fmocprotected amino acids and ethyl cyano(hydroxyimino)acetate (Oxyma Pure) were purchased from Novabiochem. Dimethylformamide (DMF) was purchased from J.T. Baker. Dichloromethane (DCM) and acetonitrile (ACN) were purchased from VWR chemical. Solvent exchange was performed in dialysis tubes from Spectrum Laboratories (cellulose ester, MWCO 500-1000 Da, 3.2 cm mL À1 ). Atto550 was purchased from ATTO-TEC GmbH. Atto550-labeled, 22 nucleotide (nt) and 100 nt DNA and their unlabelled complementary stands were purchased from Microsynth. Fetal calf serum (FCS) and phosphate buffer saline (PBS) was purchased from BioConcept. Live cell imaging solution was obtained from Thermo Fisher Scientific. Dulbecco's modified Eagle's medium (DMEM), Opti-MEM, and pen/strep were obtained from Gibco life technologies. CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Promega.

Tarvirdipour S ., Schoenenberger CA ., Benenson Y , & Palivan CG . (2020). A self-assembling amphiphilic peptide nanoparticle for the efficient entrapment of DNA cargoes up to 100 nucleotides in length. Soft matter, 16(6).

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Culturing Cells with Growth Media

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Cells were cultured in DMEM supplemented with 10% Fetal Calf Serum (FCS; Bioconcept) and 50 μg/mL of both Penicillin and Streptomycin (Sigma-Aldrich). Trypsin-EDTA (Life Technologies) was used for propagation, and frozen stocks were prepared in DMSO:FCS (1:9).

Ciuffa R., Uliana F., Mannion J., Mehnert M., Tenev T., Marulli C., Satanowski A., Keller L.M., Rodilla Ramírez P.N., Ori A., Gstaiger M., Meier P, & Aebersold R. (2022). Novel biochemical, structural, and systems insights into inflammatory signaling revealed by contextual interaction proteomics. Proceedings of the National Academy of Sciences of the United States of America, 119(40), e2117175119.

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Subcutaneous Infection of Mice with F. novicida

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Infection of mice with wild-type F. novicida strain U112 was performed at the Biozentrum, University of Basel. All animal experiments were approved (license 2535-26742, Kantonales Veterinäramt Basel-Stadt) and were performed according to local guidelines (Tierschutz-Verordnung, Basel-Stadt) and the Swiss animal protection law (Tierschutz-Gesetz). Bacteria were cultured overnight in brain heart infusion (BHI) medium (supplemented with 100 μg/mL ampicillin [AppliChem] and 0.2% L-cysteine). Bacteria were harvested by centrifugation and washed once with 1× Dulbecco’s Phosphate-Buffered Saline (DPBS). Mice were infected subcutaneously with 5 × 10³ CFU in 50 μL 1× DPBS. Infected mice had access to food and water ad libitum. Mice were sacrificed 48 hr after infection, and spleen and liver were harvested. Colony-forming units (CFUs) were determined from spleen and liver homogenates plated in serial dilutions on Mueller-Hinton agar plates supplemented with 0.1% D-glucose (Millipore), 0.1% fetal calf serum (FCS) (BioConcept), 100 μg/mL ampicillin (AppliChem), and 0.1% L-cysteine. Statistical analysis was performed using GraphPad Prism 6.

Schneider K.S., Groß C.J., Dreier R.F., Saller B.S., Mishra R., Gorka O., Heilig R., Meunier E., Dick M.S., Ćiković T., Sodenkamp J., Médard G., Naumann R., Ruland J., Kuster B., Broz P, & Groß O. (2017). The Inflammasome Drives GSDMD-Independent Secondary Pyroptosis and IL-1 Release in the Absence of Caspase-1 Protease Activity. Cell Reports, 21(13), 3846-3859.

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