Mtorfl

All animals use protocols were reviewed and approved by The University of Massachusetts Medical School Institutional Animal Care and Use Committee. Gli1^CreER (Ahn and Joyner, 2004 (link)), Gli1^LacZ (Bai et al., 2002 (link)), Villin^Cre (Madison et al., 2002 (link)), Lgr5^CreER (Barker et al., 2007 (link)), Pten^fl (Lesche et al., 2002 (link)), mTOR^fl (Risson et al., 2009 (link)), R26^mT/mG (Muzumdar et al., 2007 (link)) mice were obtained from the Jackson laboratory. The LKB1^flox (Bardeesy et al., 2002 (link)) mice were obtained from NCI mouse repository. Nkx3.2^Cre (Verzi et al., 2009 (link)) mice were kindly provided by Drs. RA Shivdasani and WE Zimmer. Cre activation of the inducible Cre lines was achieved by one-time intraperitoneal injection of 120mg/kg Tamoxifen (Sigma) at the age of one month old. Both male and female mice with appropriate genotypes were used in our study.

C57BL/6, CD45.1⁺, OT-I, OT-II, Batf3^−/−, B2m^−/−, Il12a^−/−, Mtor^fl, Map3k14^−/−, Lats1^fl, Lats2^fl, and CD11c-Cre mice were purchased from The Jackson Laboratory. Stk4^fl and Stk3^fl mice were kindly provided by Randy Johnson ^{21 (link)}, and Yap^fl and Taz^fl mice were kindly provided by Eric Olson^{22 (link)}. The mice have been backcrossed to the C57BL/6 background and were used at 8-12 weeks old. All of the genetically modified mice were viable and developed normally. For mixed bone marrow (BM) chimera generation, BM cells from WT or Mst1/2^ΔDC CD45.2.2⁺ mice were mixed with cells from CD45.1.2⁺ mice at a 1:1 ratio and transferred into lethally irradiated (11 Gy) CD45.1.1⁺ mice, followed by reconstitution for 6-8 weeks, as described previously^{23 (link)}. In certain experiments, BM cells from WT or Mst1/2^ΔDC CD45.2.2⁺ mice were transferred into lethally irradiated (11 Gy) CD45.1.1⁺ mice. For chimeras used in Fig. 2c, BM cells from WT or Mst1/2^ΔDC CD45.2.2⁺ mice were mixed with BM cells from Batf3^−/− mice at a 1:1 ratio and transferred into lethally irradiated (11 Gy) CD45.1.1⁺ mice. All mice were kept in a specific pathogen-free facility in the Animal Resource Center at St. Jude Children’s Research Hospital. Animal protocols were approved by the Institutional Animal Care and Use Committee of St. Jude Children’s Research Hospital.