Primary patient spheroids were embedded in OCT and sectioned to generate serial cuts that were numbered sequentially from 1–10. When comparing spheroids derived from different cells, section numbers were matched. Sections were fixed with 4% formaldehyde for 15 minutes and blocked with 5% BSA at 4°C overnight. Anti-human ferroportin (Amgen (38C8)) and anti-rabbit Hepcidin (Fitzgerald Industries International; cat#70R-6236) were applied for one hour followed 1:800 dilutions of rhodamine-green conjugated goat anti-human secondary antibody (Jackson ImmunoResearch, West Grove, PA, USA) for ferroportin and Alexa-fluor 555 conjugated goat anti-rabbit IgG secondary antibody (Invitrogen, Carlsbad, CA, USA) for hepcidin. Slides were mounted with ProLong Gold anti-fade reagent (Invitrogen). Images were acquired using a fluorescent inverted microscope (Zeiss Axio Vert.A1; Carl Zeiss Microscopy).
Anti rabbit hepcidin
Manufactured by Biosynth
Sourced in United States
Anti-rabbit Hepcidin is a laboratory reagent used to detect and quantify hepcidin, a key regulator of iron homeostasis, in rabbit samples. It functions as a specific antibody that binds to hepcidin, allowing for its identification and measurement.
Automatically generated - may contain errors
Lab products found in correlation
Prolong gold antifade reagent, by Thermo Fisher Scientific (2 mentions)
Anti human ferroportin, by Amgen (2 mentions)
Rhodamine green conjugated goat anti human secondary antibody, by Jackson ImmunoResearch (2 mentions)
Alexa fluor 555 conjugated goat anti rabbit igg secondary antibody, by Thermo Fisher Scientific (2 mentions)
2 protocols using anti rabbit hepcidin
1
Quantifying Ferroportin and Hepcidin in Spheroids
2
Quantifying Ferroportin and Hepcidin in Spheroids
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Primary patient spheroids were embedded in OCT and sectioned to generate serial cuts that were numbered sequentially from 1–10. When comparing spheroids derived from different cells, section numbers were matched. Sections were fixed with 4% formaldehyde for 15 minutes and blocked with 5% BSA at 4°C overnight. Anti-human ferroportin (Amgen (38C8)) and anti-rabbit Hepcidin (Fitzgerald Industries International; cat#70R-6236) were applied for one hour followed 1:800 dilutions of rhodamine-green conjugated goat anti-human secondary antibody (Jackson ImmunoResearch, West Grove, PA, USA) for ferroportin and Alexa-fluor 555 conjugated goat anti-rabbit IgG secondary antibody (Invitrogen, Carlsbad, CA, USA) for hepcidin. Slides were mounted with ProLong Gold anti-fade reagent (Invitrogen). Images were acquired using a fluorescent inverted microscope (Zeiss Axio Vert.A1; Carl Zeiss Microscopy).
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