Ca125 enzyme linked immunosorbent assay elisa kits

3-Deazaneplanocin A (3-DZNeP) was purchased from Selleckchem (Houston, TX). Antibodies to EZH2, H3K27me3, p-EGFR, p-Src, Src, p-ERK1/2, ERK1/2, p-STAT3, STAT3, p-Smad3, Smad3, Notch1, MMP2, MMP9, Histone H3 and Cleaved caspase 3 were purchased from Cell Signaling Technology (Dancers, MA). Antibodies to GAPDH, E-cadherin, p-NF-κB (p65), NF-κB (p65), Collagen I (A2), TGF-βRI, Smad7, CD68, CD31, VEGF and EGFR were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). CD3, H3K9me3, TIMP2 and Bcl-2 were purchased from Abcam (Cambridge, MA). Antibody to Bax was purchased from BD Biosciences (San Jose, CA). Antibody to β-actin was purchased from TransGen Biotech (Beijing, China). TNF-α, IL-1β, TGF-β1, MCP-1, IL-6, EZH2, VEGF, CA125 enzyme-linked immunosorbent assay (ELISA) kits were purchased from R&D Systems (Minneapolis, MN). Peritoneal dialysis solution with 4.25% glucose was purchased from Baxter Healthcare (Guangzhou, China). EZH2 siRNA was purchased from GenePharma (Shanghai, China). Lipofectamine 2000 was purchased from Invitrogen (Grand Island, NY). α-SMA, DMSO, CG, Tamoxifen and all other chemicals were obtained from Sigma-Aldrich (St. Louis, MO). Blood urea nitrogen (BUN) and glucose biochemical reagent kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).