For the analysis of human cell engraftment in humanized mice, recipient splenocytes and bone marrow cells were stained with Zombie live/dead stain (cat.no. 77184, Biolegend), anti-human CD45 (clone HI30, cat.no. 304008, 1:20 dilution, Biolegend with IgG1, PE, clone MOPC-21, cat.no. 400140 1:20 dilution, Biolegend), anti-human CD3 (clone UCHT1, cat.no. 300415, 1:20 dilution, Biolegend with IgG1, AF488, clone MOPC-21, cat.no. 400129 1:20 dilution, Biolegend), anti-human CD19 (clone HIB19, cat.no. 302228, 1:20 dilution, Biolegend with IgG1, PerCP, clone MOPC-21, cat.no. 400148 1:20 dilution, Biolegend) and anti-human CD33 (clone P67.6, cat.no. 366612, 1:20 dilution, Biolegend with IgG1, BV605, clone MOPC-21, cat.no. 400162 1:20 dilution, Biolegend). Cells were measured by flow cytometry (BD Aria and FACSDiva version 9.0 or CytExpert version 2.4 software) and gated for live cells, CD45 + cells and then followed by CD3 + cells, CD19 + or CD33 + cells. Results were expressed as percentage of positive population compared to control samples in FlowJo (BD).
Clone p67
Manufactured by BioLegend
Clone P67.6 is a monoclonal antibody that recognizes the CD67 antigen. It is commonly used for the identification and characterization of cells expressing CD67. The clone P67.6 antibody is suitable for flow cytometry and other immunoassay applications.
Automatically generated - may contain errors
2 protocols using clone p67
1
Characterizing Human Cell Engraftment in Humanized Mice
2
Immune Cell Functional Assay
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Phosphate buffered saline (PBS) pH 7.4 stock solution (10×) was purchased from Fisher Scientific (Pittsburgh, PA). NH 4 Cl, NaHCO 3 , EDTA were purchased from Sigma. PerCP/Cyanine5.5 anti-human CD33 Antibody, Mouse IgG1, κ, clone WM53 and clone P67.6 were purchased from BioLegend (San Diego, CA). Isotype control (non-specific isotype control antibody) PerCP/Cyanine5.5 Mouse IgG1, κ, Isotype Ctrl Antibody was purchased from BioLegend (San Diego, CA). Alexa Fluor® 488 anti-human CD14 antibody (Clone M5E2) was purchased from BD Pharmingen (San Diego, CA). Lithium heparin vacutainers (34 I.U.) were purchased from Becton Dickinson (Plymouth, UK). Phagotest TM and Phagoburst TM kit were purchased from Glycotope-Biotechnology (Heidelberg, Germany). All chemicals used for experimental work were of analytical grade.
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