Animals were deeply anesthetize and perfused intracardially with phosphate-buffered saline, followed by 2% paraformaldehyde and 2% glutaraldehyde in cacodylate buffer. The cochleae were resected and flushed with the fixative for 2 h at room temperature. After washing, the specimens were post-fixed for 1.5 h with 2% osmium tetroxide in phosphate buffer and then were dehydrated through a graded ethanol series and embedded in Epon. Horizontal sections of the surface of the cochlear membrane labyrinth were made, stained with uranyl acetate and lead citrate, and examined by electron microscopy (Model H-7700, Hitachi).
Model h 7700
Manufactured by Hitachi
The Hitachi Model H-7700 is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of materials at the nanoscale. It features a LaB6 electron source, a 200 kV accelerating voltage, and advanced imaging capabilities.
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Lab products found in correlation
2 protocols using model h 7700
1
Ultrastructural Analysis of Cochlear Labyrinth
2
Ultrastructural Analysis of Cell Aggregates
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Aggregates were fixed with 2% paraformaldehyde and 2% glutaraldehyde in 0.1 M cacodylate buffer for 24 hr at 4°C and postfixed for 1.5 hr with 2% osmium tetroxide in phosphate buffer. Aggregates were dehydrated through a graded ethanol series and embedded in Epon. Aggregate sections were stained with uranyl acetate and lead citrate and examined by EM (Model H-7700, Hitachi).
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