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Rabbit erk

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Rabbit ERK (Extracellular Signal-Regulated Kinase) is a laboratory equipment product from Cell Signaling Technology. It is a protein that plays a key role in the regulation of cellular processes such as cell growth, proliferation, differentiation, and survival.

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Lab products found in correlation

Bicinchoninic acid assay, by Thermo Fisher Scientific (4 mentions) Il 12, by Abcam (2 mentions) Rabbit p erk, by Cell Signaling Technology (2 mentions) Rabbit p akt, by Abcam (2 mentions) Rabbit jnk, by Abcam (2 mentions) Rabbit p jnk, by Abcam (2 mentions) Rabbit il 10, by Abcam (2 mentions) Rabbit β tubulin, by Merck Group (2 mentions) Rabbit akt, by Abcam (1 mentions) Sds polyacrylamide gel electrophoresis, by Thermo Fisher Scientific (1 mentions) Sds page, by Thermo Fisher Scientific (1 mentions) Rabbit anti jnk, by Cell Signaling Technology (1 mentions) Rabbit anti phospho akt ser473, by Cell Signaling Technology (1 mentions) Rabbit anti phospho p38, by Cell Signaling Technology (1 mentions) Rabbit anti p38, by Cell Signaling Technology (1 mentions) Rabbit anti mmp 2, by Cell Signaling Technology (1 mentions) Rabbit anti mmp 9, by Cell Signaling Technology (1 mentions) Alexa 488 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Gel electrophoresis reagents, by Bio-Rad (1 mentions) Rabbit anti myc, by Merck Group (1 mentions)

4 protocols using rabbit erk

1

Characterization of Macrophage and Smooth Muscle Cell Signaling

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Peritoneal macrophages, RAW 264.7 and hASMCs were homogenized in RIPA lysis buffer. Protein concentrations were determined through the Bicinchoninic Acid assay (Thermo Scientific) according to the manufacturer’s protocol. SDS–polyacrylamide gel electrophoresis (Invitrogen) was performed using 10 μg of protein per well. Western blot was performed with the following primary antibodies: rabbit Chi3l1 (dilution 1:1,000), rabbit Akt (1:500), rabbit p-Akt (1:500), rabbit JNK (1:3,000), rabbit p-JNK (1:3,000) and rabbit IL-10 (1:1,000; all obtained from Abcam), and IL-12 (1:1,000), rabbit ERK (1:3,000) and rabbit p-ERK (1:3,000; all obtained from Cell Signaling), and rabbit β-tubulin (1:1,000; obtained from Sigma-Aldrich; Supplementary Fig. 10).
Maegdefessel L., Spin J.M., Raaz U., Eken S.M., Toh R., Azuma J., Adam M., Nagakami F., Heymann H.M., Chernugobova E., Jin H., Roy J., Hultgren R., Caidahl K., Schrepfer S., Hamsten A., Eriksson P., McConnell M.V., Dalman R.L, & Tsao P.S. (2014). miR-24 limits aortic vascular inflammation and murine abdominal aneurysm development. Nature Communications, 5, 5214.
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2

Protein Expression and Signaling Pathways

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Peritoneal macrophages, RAW 264.7, and hASMCs were homogenized in RIPA lysis buffer. Protein concentrations were determined through the Bicinchoninic Acid assay (ThermoScientific), according to the manufacturer's protocol. SDS-PAGE (Invitrogen) was performed using 10µg of protein per well. Western blot was performed with the following primary antibodies: rabbit Chi3l1 (dilution 1:1000), rabbit Akt (1:500), rabbit p-Akt (1:500), rabbit JNK (1:3000), rabbit p-JNK (1:3000), rabbit IL-10 (1:1000; all from Abcam) and IL-12 (1:1000), rabbit ERK (1:3000), rabbit p-ERK (1:3000; all from Cell Signaling) and rabbit β-tubulin (1: 1000; both Sigma Aldrich; Supplementary Fig. 10).
Maegdefessel L., Spin J.M., Raaz U., Eken S.M., Toh R., Azuma J., Adam M., Nagakami F., Heymann H.M., Chernugobova E., Jin H., Roy J., Hultgren R., Caidahl K., Schrepfer S., Hamsten A., Eriksson P., McConnell M.V., Dalman R.L, & Tsao P.S. (2014). miR-24 limits aortic vascular inflammation and murine abdominal aneurysm development. Nature Communications, 5, 5214.
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3

Western Blot Antibody Reagents

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Rabbit anti-WAVE3 (1∶1000), rabbit anti-MMP9 (1∶1000), rabbit anti-MMP2 (1∶1000), rabbit anti-p38 (1∶1000), rabbit anti phospho p38 (1∶1000), rabbit phospho ERK (1∶1000), rabbit ERK (1∶1000), rabbit anti phospho AKT (Ser473; 1∶1000), rabbit anti panAKT (1∶1000), rabbit anti Phospho JNK (1∶1000), rabbit anti-JNK (1∶1000), mouse anti-phospho p65 (Ser536) (1∶1000), rabbit anti-Cortatcin are from Cell Signaling Technologies. (Danvers, MA); mouse anti-GFP, mouse anti-WAVE2 (1∶3000), mouse anti-WAVE1 (1∶3000) are from Santa Cruz Biotechnology Inc (Santa Cruz, CA); rabbit anti-NFκB p65 (1∶5000) from Biolegend (San Diego, CA), rabbit anti NAP1 (1∶2000), rabbit anti ABI1 (1∶5000), rabbit anti CYFIP2 (1∶3000), mouse anti-actin (1∶5000), rabbit anti-Myc (1∶1000) are from Sigma-Aldrich (St. Louis, MO); goat horseradish peroxidase-conjugated anti-mouse IgG (1∶5000) and goat horseradish peroxidase-conjugated anti-rabbit IgG (1∶5000) from Calbiochem; and Alexa 488-conjugated anti-rabbit IgG and Alexa 568-conjugated anti-mouse IgG, Alexa 568-conjugated phalloidin are from Invitrogen. Vecta-shield with 4′,6-diamidino-2-phenylindole was from Vector Laboratories (Burlingame, CA). Gel electrophoresis reagents were from Bio-Rad (Hercules, CA).
Davuluri G., Augoff K., Schiemann W.P., Plow E.F, & Sossey-Alaoui K. (2014). WAVE3-NFκB Interplay Is Essential for the Survival and Invasion of Cancer Cells. PLoS ONE, 9(10), e110627.
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4

Western Blotting Protocol for Fly Eyes

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Western blotting was performed as described [39 (link)]. Briefly, 20 fly eyes were dissected and homogenized in SDS sample buffer with a Pellet Pestle (Kimble/Kontes). The proteins were fractionated by SDS-PAGE and transferred to Immobilon-P transfer membranes (Millipore) in Tris-glycine buffer. The blots were probed with Mouse Tubulin primary antibodies (1:2000 dilution, Developmental Studies Hybridoma Bank), Rabbit ERK (1:1000, Cell Signaling Technology), Mouse β-Actin primary antibodies (1:1000 dilution, Santa Cruz Biotechnology) and Rabbit RACK1 antibodies (1:1000 dilution, Dr. J. Kadrmas lab) followed by IRDye 680 goat anti-Rabbit IgG (LI-COR) and IRDye 800 goat anti-Mouse IgG (LI-COR) as the secondary antibodies. The signals were detected with the Odyssey infrared imaging system (LI-COR).
Xie J., Han Y, & Wang T. (2021). RACK1 modulates polyglutamine-induced neurodegeneration by promoting ERK degradation in Drosophila. PLoS Genetics, 17(5), e1009558.
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