Total RNA from RPE-1 cells was purified using RNeasy (74104; Qiagen). mRNA levels were analyzed by quantitative RT-PCR procedures using the Super Script III First-Strand Synthesis SuperMix kit (PN 11752250; Life Technologies) and the qPCR FAST Sybr Green PCR Master Mix kit (PN 4367659; Applied Biosystems) in an ABI 7900HT apparatus. The results were normalized with respect to the expression of Gapdh mRNA in the same samples. As indicated in the corresponding figure legend, three to five independent experiments were performed in triplicate. Data were analyzed with GenEX software. The oligonucleotide primers used for quantitative PCR are listed in Table S1.
Qpcr fast sybr green pcr master mix kit
Manufactured by Thermo Fisher Scientific
The QPCR FAST Sybr Green PCR Master Mix kit is a ready-to-use solution for performing quantitative real-time PCR (qPCR) reactions. It contains all the necessary components, including a proprietary Sybr Green I dye, for the amplification and detection of target DNA sequences.
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Lab products found in correlation
2 protocols using qpcr fast sybr green pcr master mix kit
1
Quantitative RT-PCR Analysis of RPE-1 Cells
2
Quantifying mRNA Levels in MDCK Cells
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Total RNA from MDCK cells was purified using RNeasy (Qiagen). MAL and E-cadherin mRNA levels were quantified by qRT-PCR procedures using the Super Script III First-Strand Synthesis SuperMix kit (Life Technologies, PN 11752250) and the qPCR FAST Sybr Green PCR Master Mix kit (Applied Biosystems, PN 4367659) in an ABI 7900HT equipment. The results were normalized with respect to the expression of β-actin, caveolin-1 and glyceraldehyde 3-phosphate dehydrogenase mRNA in the same samples. Data were analyzed with GenEX software.
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