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640 ir ft ir spectrometer

Manufactured by Agilent Technologies
Sourced in United States

The 640-IR FT-IR spectrometer is a compact and versatile Fourier Transform Infrared (FT-IR) spectrometer designed for a wide range of analytical applications. It utilizes a step-scan interferometer to provide high-quality infrared spectroscopic data for qualitative and quantitative analysis.

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4 protocols using 640 ir ft ir spectrometer

1

Characterization of Fe3O4@CaCO3 Nanocomposite

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Dynamic light scattering (DLS) and zeta potential (ζ-potential) measurements were carried out on Malvern Zetasizer Nano (Malvern Instrument Ltd., Worcestershire, UK). For DLS studies, Fe3O4@CaCO3 was diluted in deionized water to a concentration of 100 μg/mL. Transmission electron microscopy (TEM) images were obtained on a JEM-1400 (Jeol, Tokyo, Japan). Images were captured using a side-mounted Veleta digital camera (EM SIS, Muenster, Germany). UV-vis spectra were recorded on a UV-2100 spectrophotometer (Shimadzu, Kyoto, Japan) and microplate reader Clariostar (BMG, Ortenberg, Germany). FTIR spectra were measured on a 640-IR FT-IR spectrometer (Varian, MA, USA) from 4000 to 400 cm−1 at room temperature accompanied with a KBr pellet.
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2

FTIR Analysis of Compound Complexes

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FTIR spectra were obtained with a Varian 640-IR FTIR® spectrometer (Varian Inc., Palo Alto, CA, USA), equipped with a universal ATR sampling accessory. A small quantity of the individual compounds (PSO, 5-MOP, and HP-β-CD) or PSO-HP-β-CD and 5-MOP-HP-β-CD inclusion complex (1:1) and physical mixtures were placed on the diamond ATR crystal. Analysis was performed over a wavelength ranging from 4000 to 400 cm−1 with a resolution of 4 cm−1.
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3

Spectroscopic Characterization of Chemical Compounds

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Melting points (uncorrected) were determined on a Stuart™ SMP40 automatic melting point apparatus. 1H and 13C NMR spectra were recorded on a Bruker Fourier 300 spectrometer (300 MHz), using DMSO-d6 as a solvent and TMS as an internal reference. IR spectra were recorded on a Varian 640-IR FT-IR spectrometer using KBr mode. Microwave-assisted reactions were performed in closed vessel focused single mode using a CEM Discover SP microwave synthesizer (CEM, USA). The reaction temperatures were measured by an equipped IR sensor.
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4

Physicochemical Characterization of AuNPs

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Nanoparticle size, charge and polydispersity were determined by dynamic light scattering (DLS) using a Malvern Nano Zetasizer ZS Series. Fourier transform infrared spectroscopy (FTIR) spectra were recorded in the range of 400-4000 cm -1 at a resolution of 4 cm -1 on a Varian 640-IR FTIR spectrometer. UV-Visible spectra were recorded using a Perkin Elmer Lambda 35 in the range 400-600 nm at 0.1 nm increments. Stress testing AuNP stability in high ionic solutions (1 M NaCl) was conducted by UV/Vis measurement of the characteristic plasmon peak over 24 h. Serum stably was established using DLS following a 24 h incubation medium containing 10% or 40% foetal calf serum.
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