4 line laser unit

Manufactured by Nikon

The 4 line laser unit is a precision instrument designed for a variety of applications. It generates four parallel laser beams, providing a stable and consistent light source. The core function of this product is to deliver accurate and reliable laser outputs for various measurement, alignment, and calibration tasks.

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Lab products found in correlation

Antifade mounting medium, by Vector Laboratories (1 mentions) A1rsi, by Nikon (1 mentions) A1rsi inverted confocal microscope, by Nikon (1 mentions)

2 protocols using 4 line laser unit

Confocal Microscopy of Drosophila Eye

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Specimens were mounted in VECTASHIELD Antifade Mounting Medium. Specimens were mounted so that the eye imaginal disk is on the top next to the cover slip, and the optic lobe is on the bottom. Images were obtained on a Nikon A1R-Si inverted confocal microscope with 4 line laser unit (405/488/561/640) and with a 60X oil objective. Z stacks were acquired with a step size of 0.125 μm between optical sections.

Ji W., Wu L.F, & Altschuler S.J. (2021). Analysis of growth cone extension in standardized coordinates highlights self-organization rules during wiring of the Drosophila visual system. PLoS Genetics, 17(11), e1009857.

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Confocal Microscopy Imaging Protocol

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Samples were mounted in VECTASHIELD Antifade Mounting Medium. Images were obtained on a Nikon A1R-Si inverted confocal microscope with 4 line laser unit (405/488/561/640) and with a 60X oil objective. Z stacks were acquired with a step size of 0.125 μm between optical sections.

Ji W., Wu L.F., & Altschuler S.J. (2020). On the role of photoreceptor identity in controlling accurate wiring of theDrosophilavisual circuit.

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