Activated charcoal powder

Manufactured by Merck Group

Sourced in United States, Germany

Activated charcoal powder is a fine, black powder produced by heating carbon-rich materials, such as wood or coal, in the absence of oxygen. The porous structure of activated charcoal gives it a large surface area, which allows it to effectively adsorb a variety of substances, including gases, liquids, and dissolved organic compounds.

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5 protocols using activated charcoal powder

FBS Charcoal Stripping Protocol

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To remove residual VitD in the culture medium, fetal bovine serum (FBS, Thermo Scientific) was subjected to stripping according to literature 25 (link). Briefly, FBS was incubated with 2.5% activated charcoal powder (Sigma-Aldrich) and incubate at 4 °C overnight with gentle shaking. FBS was then centrifuged at 2000g at 4 °C for 15 min. Supernatant (C-FBS) was collected, filtered through a 0.22μm syringe filter and stored at -20 °C.

Li N., Yao M., Liu J., Zhu Z., Lam T.L., Zhang P., Kiang K.M, & Leung G.K. (2022). Vitamin D Promotes Remyelination by Suppressing c-Myc and Inducing Oligodendrocyte Precursor Cell Differentiation after Traumatic Spinal Cord Injury. International Journal of Biological Sciences, 18(14), 5391-5404.

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Preparation and Characterization of Fluorescent Polymeric Nanoparticles

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The following were purchased from Sigma Aldrich: polyethylene glycol) methyl ether MW 750, methanesulfonyl chloride, triethylamine, potassium carbonate, thioacetic acid, 0.5 M sodium methoxide solution, propylene sulfide, acetic acid, calcein, ethyl eosin, dichloromethane, Celite filter cel, activated charcoal powder, anhydrous tetrahydrofuran, tetrahydrofuran, ethanol, methanol, diethyl ether, deuterated chloroform, and sepharose CL-6B. The following reagents were purchased from ThermoFisher Scientific: tetramethylrhodamine-dextran 70 kDa, Texas red-dextran 10 kDa, DiD, Texas red conjugated ovalbumin, Lysotracker green, DAPI, toluene, hexanes, and phosphate buffered saline (1× PBS) tablets (product BP2944). Monophosphoryl lipid A (MPL) from Salmonella minnesota R595 was purchased from Invivogen. All antibodies and flow cytometry reagents were purchased from BioLegend.

Bobbala S., Allen S.D, & Scott E.A. (2018). Flash nanoprecipitation permits versatile assembly and loading of polymeric bicontinuous cubic nanospheres. Nanoscale, 10(11), 5078-5088.

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Pharmacological Characterization of Compounds

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Atropine, potassium chloride, acetylcholine, verapamil hydrochloride, activated charcoal powder, ethanol, methanol, papaverine, omeprazole, and loperamide hydrochloride were obtained from Sigma-Aldrich (Co, St Louis, MO, USA). Castor oil was purchased from KCL Pharma (Karachi, Pakistan).

Ansari S.F., Khan A.U., Qazi N.G., Shah F.A, & Naeem K. (2021). Proteomic Analysis and In Vivo Studies Reveal the Potential Gastroprotective Effects of CHCl3 and Aqueous Extracts of Ficus palmata. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 6613140.

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Synthesis of ZnO-Activated Charcoal Composite

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ZnO powder (99.99%) was purchased from Sigma-Aldrich. Activated charcoal powder and ethanol were purchased from Merck Company.

Yadav K., Mehta B.R., Bhattacharya S, & Singh J.P. (2016). A fast and effective approach for reversible wetting-dewetting transitions on ZnO nanowires. Scientific Reports, 6, 35073.

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Sustainable Biorefinery Feedstock Sourcing

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Isopropyl alcohol was purchased from Honeywell (Hanover, Germany). Petroleum solvent, Nefras grade C2-80/120, was purchased from “Latvijas ķīmija” (Riga, Latvia). Activated charcoal powder, chloroform-d, Kraft lignin, and beechwood xylan were purchased from Merck KGaA (Darmstadt, Germany). Nanofibrillated cellulose (NFC) was produced from old filter paper waste according to the method previously reported by the authors [27 (link)]. NFC sizes ranged from 40 to 120 nm in diameter, and fiber lengths from around 500 to 2000 nm. Pine needles (Pinus sylvestris, L.) were gathered locally in Latvia through a forestry company.

Beluns S., Platnieks O., Sevcenko J., Jure M., Gaidukova G., Grase L, & Gaidukovs S. (2022). Sustainable Wax Coatings Made from Pine Needle Extraction Waste for Nanopaper Hydrophobization. Membranes, 12(5), 537.

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