Monoclonal rabbit antibodies against sirt6

TRIzol was purchased from Sigma (St Louis, Missouri, USA). TMR red-labeled In-Situ Cell Death Detection reagent and sTREM-1, the sSirt6 enzyme-linked immune sorbent assay (ELISA) kit were purchased from Roche Applied Science (Indianapolis, USA). Monoclonal antibodies against CD62-PE, CD31-PE, CD42-FITC, and IgG-PE were purchased from BD (Shanghai, China), and monoclonal rabbit antibodies against Sirt6, TREM-1, LC3, BECN1, LAMP2, p62, Caspase-1, and GAPDH were purchased from Cell Signaling (Denver, Colorado, USA). Fetal bovine serum (FBS) and Lipofectamine^® 2000 Transfection Reagent were purchased from Invitrogen (Carlsbad, CA, USA). The cDNA Synthesis Kit and Premix Ex Taq SYBR Green PCR Kit were purchased from Takara (Shiga, Japan). The adv-Sirt6, LV-Sirt6 shRNA, adv-TREM-1, LV-TREM-1 shRNA, LV-LC3 shRNA, and GFR, GRP double labeled adv-LC3 were purchased from HanBio. (Shanghai, China). Other unmentioned reagents were purchased from Shenggong Bio. (Shanghai, China).

Glucose and TRIzol were purchased from Sigma (St Louis, Missouri, USA). Monoclonal antibodies against CD62-PE, CD31-PE, CD42-FITC and IgG-PE were obtained from BD (Shanghai, China), and the monoclonal rabbit antibodies against Sirt6, p-eNOS, eNOS and β-actin were obtained from Cell Signaling (Denver, Colorado, USA). The fetal bovine serum (FBS), DMEM culture medium with or without glucose, Lipofectamine® 2000 Transfection Reagent and ECMatirm™ solution were purchased from Invitrogen (Carlsbad, CA, USA). The cDNA Synthesis Kit and Premix Ex Taq SYBR Green PCR Kit were purchased from Takara (Shiga, Japan). The adv-Sirt6, Sirt6 siRNA, CMV-Sirt6 shRNA, Sirt1 siRNA and Drosha siRNA were obtained from HanBio. (Shanghai, China). Other unmentioned reagents were purchased from Shenggong Bio. (Shanghai, China).