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4 protocols using ampicillin a9518

1

Molecular Biology Reagents and Protocols

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Designed oligonucleotides and DNA extraction kits (product codes: 27104 and 28104) were ordered from Qiagen. Expand High fidelity Taq polymerase (11732641001), T4 DNA ligase (10716359001), HindIII (10656321001), and BamHI (10 656 275 001) were from Roche Diagnostics (Mannheim, Germany). NdeI (R0111S) was from New England Biolabs and the dNTP mix (18427013) from Invitrogen. Tryptone (211921), yeast extract (212750), and sodium (2-sulfonatoethyl)-methanethiosulfonate (MTSES, AM3720 INTERCHIM) were from Thermo Fisher. Amberlite XAD-4 (06444), ampicillin (A9518), bathophenanthroline (146617), diamide (D3648), dithioerythritol (D8255), egg yolk phospholipids (lecithin from eggs, 61755), Na2SO4 (239313), N-dodecanoylsarcosine (sarkosyl, L512), N,N′-1,2-phenylenedimaleimide (o-PDM, 104590), N,N′1,3-phenylenedimaleimide (m-PDM, 160458), PIPES (P6757), phenylarsine oxide (PAO, P3075) pyridoxal-5’-phosphate (82870), sephadex G-75 (17-0050-01), and Tris base (10708976001) triton X-114 (93422) were from Sigma-Aldrich. The radioactive substrates 2-Oxo [1-14C]glutaric acid (NEC597) and 14C-NAD+ (NEC831) were purchased from Perkin Elmer, and [8-3H]GTP (TRK314) from Amersham Biosciences. All reagents were of analytical grade.
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Characterization of Gold Nanoparticles

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Gold (III) chloride hydrate (50790), Gold nanoparticles (Uncapped and dissolved in PBS, catalogue no. 752584), ampicillin (A 9518) and kanamycin (60615), XTT (X4626) were purchased from Sigma-Aldrich. Luria-Bertini (LB) medium, yeast extract, peptone, dextrose and agar used for culture growth were purchased from Himedia.
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Antibiotic Procurement Protocol

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Ampicillin (A9518), kanamycin (127K0058), and streptomycin (081K1275) were obtained from Sigma Aldrich.
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4

Antimicrobial Susceptibility of PAO1 and MRSA

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Pseudomonas aeruginosa PAO1 (H103 wild type) and methicillin-resistant Staphylococcus aureus (MRSA; C623) (Cherkasov et al., 2009) were obtained from the Department of Science and Environment, Roskilde University, Denmark. Ampicillin (A9518) was purchased from Sigma Aldrich (Brøndby, Denmark).
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