Samples were diluted by addition of 2X Novex Tricine SDS Sample Buffer and boiled at 85° C for two minutes. The denatured protein was loaded alongside an EZ-Run Protein Ladder (Fisher BioReagents, Fair Lawn, NJ) on a Novex 10 – 20 % Tricine gradient gel and electrophoresed using Novex Tricine SDS Running Buffer for 90 minutes at 125 V. The gel was then stained with Coomassie Brilliant Blue R-250 and analyzed with a Bio Rad ChemiDoc imaging system.
Ez run protein ladder
Manufactured by Thermo Fisher Scientific
The EZ-Run protein ladder is a pre-stained protein standard used for molecular weight determination in SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) analysis. It provides a reference for estimating the molecular weights of unknown protein samples.
Automatically generated - may contain errors
Lab products found in correlation
Texas red dextran, by Thermo Fisher Scientific (2 mentions)
Iodoacetamide alkyne, by Thermo Fisher Scientific (2 mentions)
Ianbd, by Thermo Fisher Scientific (2 mentions)
Ni2 nitrilotriacetate ni nta resin, by Qiagen (2 mentions)
Blue oxazine, by Eurogentec (2 mentions)
Red oxazine, by Eurogentec (2 mentions)
Nile blue, by Eurogentec (2 mentions)
Chromis dyes chromis 630 and 678, by Eurogentec (2 mentions)
Click it protein reaction buffer kit, by Thermo Fisher Scientific (2 mentions)
Chemidoc imaging system, by Bio-Rad (1 mentions)
Xl10 gold, by Thermo Fisher Scientific (1 mentions)
Fluo 3, by Thermo Fisher Scientific (1 mentions)
Gcamp6f, by Addgene (1 mentions)
Bl21 gold de3, by Thermo Fisher Scientific (1 mentions)
T4 dna ligase, by Thermo Fisher Scientific (1 mentions)
Restriction enzyme, by New England Biolabs (1 mentions)
4 protocols using ez run protein ladder
1
Protein Denaturation and Visualization
2
Plasmid-based Fluorescent Biosensors
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GCaMP6f, G-GECO 1.0 and GEM-GECO plasmids were purchased from Addgene (plasmid #40755, #32466 and #32463, respectively). pET41a and pEGFP-N1 vectors were obtained from Novagen and Clontech, respectively. XL10-Gold and BL21 (DE3) Gold cells were purchased from Invitrogen. Fluo-3 was purchased from Molecular Probes and EZ-Run protein ladder from Fisher Bioreagents. Restriction enzymes were obtained from New England Biolabs and T4 DNA ligase from Fermentas. Br2-BAPTA was purchased from Life Technologies.
3
Fluorescent Protein Labeling Strategies
4
Fluorescent Protein Labeling Strategies
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Escherichia coli XL10-Gold and BL21(DE3) Gold cells were purchased from Stratagene. EZ-Run protein ladder was purchased from Fisher Bioreagents and Ni2+-nitrilotriacetate (Ni-NTA) resin from Qiagen. IANBD, Click-iT® Protein Reaction Buffer kit and iodoacetamide alkyne were obtained from Life Technologies. BADAN, oxazine derivatives (Blue Oxazine, Red Oxazine and Nile Blue) and Chromis dyes (Chromis 630 and 678) were purchased from Eurogentec, Chromeon and Cyanagen, respectively. The merocyanine dyes (Mero 53, 62 and 87) were synthesized as previously described [10 (link)–12 (link)]. Texas Red–dextran was obtained from Life Technologies.
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