Human skin stem cells (SSCs) were obtained from biopsies of adult male and female patients after Ethical Committee approval (Ethical Clearance Nos. 0021565/2018 and 22/03/2018—Commissione Etica CNR). They were isolated and cultured as previously described [50 (link)]. The positive selection of stem cells from skin was obtained with magnetic cell sorting using a primary monoclonal anti-c/kit (CD117) antibody (Miltenyi Biotec, Minneapolis, MN, USA). Cells showed positivity for all mesenchymal surface markers (CD73, CD90, and CD105) and were negative for CD31 and CD45, as previously described [50 (link)].
Human skin fibroblast 1 (HFF1)(HFF-1(ATCC SCRC-1041)) cells were purchased from ATCC (Manassas, VA, USA) and cultured in a low-glucose Dulbecco’s modified Eagle’s Medium (DMEM) (Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS Life Technologies), 2 mM l-glutamine (Euroclone, Milano, Italy), and 1% penicillin/streptomycin (Euroclone) [70 (link)].
Human skin fibroblast 1 (HFF1)(HFF-1(ATCC SCRC-1041)) cells were purchased from ATCC (Manassas, VA, USA) and cultured in a low-glucose Dulbecco’s modified Eagle’s Medium (DMEM) (Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS Life Technologies), 2 mM l-glutamine (Euroclone, Milano, Italy), and 1% penicillin/streptomycin (Euroclone) [70 (link)].