Claudin 3
Manufactured by Cell Signaling Technology
Sourced in United States
Claudin 3 is a tight junction protein that plays a key role in regulating the permeability of epithelial and endothelial cell layers. It is a member of the claudin family of proteins and is involved in the formation and maintenance of tight junctions between cells.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Cell Signaling Technology (2 mentions)
Occludin, by Cell Signaling Technology (2 mentions)
Clostridium butyricum, by China General Microbiological Culture Collection Center (1 mentions)
Mpo kit, by Nanjing Jiancheng (1 mentions)
Tissue protein extract, by Thermo Fisher Scientific (1 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Crystal violet stain solution 1, by Solarbio (1 mentions)
Tnf α and il 1β elisa kits, by BioLegend (1 mentions)
2 protocols using claudin 3
1
Bacterial Culture and Antibody Procurement
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Escherichia coli O111:K58 (CVCC1450) was obtained from the China Institute of Veterinary Drug Center (Beijing, China) and cultured in nutrient Luria-Bertani (LB) broth (Sigma-Aldrich L3022, Burlington, MA, USA) overnight at 37°C with shaking. Clostridium butyricum (ATCC 19398) was obtained from the China General Microbiological Culture Collection Center and cultured in sterile Reinforced Clostridial Medium at 37°C under anaerobic conditions until the logarithmic phase of growth with a bacterial density of 0.5 at A600. Primary antibodies for ZO-1, occludin, claudin 3, TLR4, β-actin, and phosphorylated and nonphosphorylated forms of NF-κB were purchased from Cell Signaling Technologies, Inc. (Beverly, MA, USA). Horseradish peroxidase (HRP) secondary antibody and the primary antibody of H3, Ac-H3, were purchased from GeneTex (Irvine, CA, USA).
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2
Murine Mastitis Model Optimization
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TNF-α and IL-1β ELISA kits were purchased from Biolegend (CA, USA). Tissue protein extract and BCA Protein Assay Kit were bought from Thermo (Thermo, MA, USA). Trypticase Soy Broth (TSB) and bacillus medium were purchased from Qingdao Haibo Biotechnology Co., Ltd (Qingdao, China). Crystal Violet Stain solution, 1% was acquired from Solarbio (Solarbio, Beijing, China). All the monoclonal antibodies, including β-actin, p65, IκB, the phosphorylation of p65, phosphorylation IκB, ZO-1, Occludin, and Claudin-3 were recruited from Cell Signaling Technology (Beverly, MA, USA). MPO kit was available from Nanjing Jiancheng Co., Ltd (Nanjing, China). Animals A total of 60 Balb/c mice (40 females and 20 males) aged 6-8 weeks were purchased from the Liaoning Changsheng Biotechnology Co. Ltd. The animal experiments were subject to approval by the Animal Ethics Committee of Jilin University (KT202103058). Females and males are mixed in miniature isolation cages in about a ratio of 2 to 1 after adapting to the environment with free food and water. The mastitis model was established complying with the experimental animal manual. This study is built on the Handbook on the Care and use of Experimental Animals published by the National Institutes of Health.
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