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3 protocols using ciprofloxacin hydrochloride monohydrate

1

Antimicrobial Susceptibility Testing of Klebsiella and E. coli

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K. pneumoniae and E. coli strains were purchased from the American Type Culture Collection (Manassas, VA) or the National Collection of Type Cultures (London, United Kingdom). Cation-adjusted Mueller-Hinton broth was used for antibacterial susceptibility testing and time-kill assays, and heart infusion agar was used for the enumeration of viable cells.
TP0586532 was synthesized by the Department of Medicinal Chemistry, Taisho Pharmaceutical Co., Ltd. (Saitama, Japan). Amikacin disulfate salt, colistin sulfate, piperacillin, tigecycline hydrate, and azithromycin dihydrate were purchased from Sigma-Aldrich (St. Louis, MO). Cefepime hydrochloride was purchased from US Pharmacopeia (Rockville, MD). Meropenem trihydrate were purchased from US Pharmacopeia or FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Ciprofloxacin hydrochloride monohydrate was purchased from FUJIFILM Wako Pure Chemical Corporation.
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2

Antimicrobial Susceptibility Testing of Klebsiella and E. coli

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K. pneumoniae and E. coli strains were purchased from the American Type Culture Collection (Manassas, VA) or the National Collection of Type Cultures (London, United Kingdom). Cation-adjusted Mueller-Hinton broth was used for antibacterial susceptibility testing and time-kill assays, and heart infusion agar was used for the enumeration of viable cells.
TP0586532 was synthesized by the Department of Medicinal Chemistry, Taisho Pharmaceutical Co., Ltd. (Saitama, Japan). Amikacin disulfate salt, colistin sulfate, piperacillin, tigecycline hydrate, and azithromycin dihydrate were purchased from Sigma-Aldrich (St. Louis, MO). Cefepime hydrochloride was purchased from US Pharmacopeia (Rockville, MD). Meropenem trihydrate were purchased from US Pharmacopeia or FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Ciprofloxacin hydrochloride monohydrate was purchased from FUJIFILM Wako Pure Chemical Corporation.
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3

Antimicrobial Agents Susceptibility Assay

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The following antimicrobial agents were used: cefmetazole (sodium salt, Sigma-Aldrich Japan); clindamycin phosphate (MP Bio Japan, Tokyo, Japan); ofloxacin (LKT Laboratories, St. Paul, MN, USA); and amoxicillin trihydrate, azithromycin, clarithromycin, ciprofloxacin hydrochloride monohydrate, and cefcapene pivoxil hydrochloride hydrate (all from Wako Pure Chemical Industries, Osaka, Japan). Stock solutions were made by dissolving each antibiotic in purified water or ethanol; 4-fold serial dilutions of each stock were prepared, and each solution was filter-sterilized (pore size, 0.22 μm; Millipore, Bedford, MA, USA). Solutions were added to blood agar to yield final antibiotic concentrations of from 32 to 0.0019 μg/ml.
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