Eclipse cyclotron

Manufactured by Siemens

The Eclipse cyclotron is a compact particle accelerator designed for the production of medical radioisotopes. It uses a magnetic field and high-frequency electric fields to accelerate charged particles, typically protons or deuterons, to high energies. The core function of the Eclipse cyclotron is to generate these accelerated particles for use in various applications, such as the synthesis of radioactive tracers for medical imaging and therapy.

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Lab products found in correlation

Anhydrous dimethylformamide, by Thermo Fisher Scientific (1 mentions) Model 996, by Waters Corporation (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

Eclipse HP cyclotron (3 citations) RDS eclipse RD/HP medical cyclotron (2 citations)

2 protocols using eclipse cyclotron

HPLC Purity Determination and Radiolabeling

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The purity of all final compounds was determined by HPLC analysis and showed purity >95%. Compound 1 (CF53) was purchased form MedChemExpress; anhydrous dimethylformamide (DMF) was purchased from Acros Organics. The analytical separation was conducted on an Agilent 1100 series HPLC fitted with a diode-array detector, quaternary pump, vacuum degasser, and autosampler. Mass spectrometry data were recorded on an Agilent 6310 ion trap mass spectrometer (ESI source) connected to an Agilent 1200 series HPLC with a quaternary pump, vacuum degasser, diode-array detector, and autosampler.
[¹¹C]CO₂ was produced via the ¹⁴N (p, α) ¹¹C reaction on nitrogen with 2.5% oxygen, with 11 MeV protons (Siemens Eclipse cyclotron), and trapped on molecular sieves in a TRACERlab FX-MeI synthesizer (General Electric). [¹¹C]CH₄ was obtained by the reduction of [¹¹C]CO₂ in the presence of Ni/hydrogen at 350°C and recirculated through an oven containing I₂ to produce ¹¹CH₃I via a radical reaction.
All animal studies were carried out at Massachusetts General Hospital (PHS Assurance of Compliance No. A3596-01). The Subcommittee on Research Animal Care (SRAC) serves as the Institutional Animal Care and Use Committee (IACUC) for the Massachusetts General Hospital (MGH). SRAC reviewed and approved all procedures detailed in this paper.

Bai P., Lan Y., Wang H., Chen Z., Fiedler S., Striar R., Lu X, & Wang C. (2020). Development of a Novel Positron Emission Tomography (PET) Radiotracer Targeting Bromodomain and Extra-Terminal Domain (BET) Family Proteins. Frontiers in Molecular Biosciences, 7, 198.

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Radiosynthesis and Evaluation of [11C]MPC-6827

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All commercial chemicals and solvents used in the synthesis were purchased from Sigma–Aldrich Chemical Co., (St. Louis, MO), or Fisher Scientific Inc., (Springfield, NJ) and were used without further purification. Gamma-ray detector (Bioscan Flow-Count fitted with a NaI detector) coupled in series with the UV detector (Waters Model 996 set at λ 254 nm) was used for detection of radiolabeled products. Data acquisition for both the analytical and preparative systems was accomplished using a Waters Empower Chromatography System. MPC-6827 and desmethyl-MPC-6827 were synthesized according as previously reported [14 (link)]. [¹¹C]CO₂ was produced from a Siemens Eclipse cyclotron. Radiosynthesis of [¹¹C]MPC-6827 was achieved by reacting corresponding desmethyl-MPC-6827 phenolate with [¹¹C]CH₃I in a GE-FX2MeI/FX2M radiochemistry module using our established procedure [14 (link)–16 (link)]. All animal experimental procedures were carried out in accordance with the Institutional Animal Care Committee ethical guidelines of Columbia University Medical Center. All experiments were performed in male C57BL/6 J mice (Jackson Laboratories, Bar Harbour, ME) during adolescence and adulthood (postnatal day 35–120).

Kumar J.S., Molotkov A., Salling M.C., Carberry P., Prabhakaran J., Castrillon J, & Mintz A. (2021). In vivo evaluation of a microtubule PET ligand, [11C]MPC-6827, in mice following chronic alcohol consumption. Pharmacological reports : PR, 74(1), 241-247.

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