Total RNA was isolated from LDM using the Tri-Reagent and RNeasy Mini kit (Qiagen) with an on-column DNase treatment according to the manufacturer’s protocol. The RNA integrity was assessed on a 1% agarose gel by electrophoresis. The RNA concentration was measured by a Nano Drop ND-1000 Spectrophotometer (PEQLAB). The quality and quantity of small RNA were assessed with an Agilent 2100 Bioanalyzer (Agilent) using an Agilent small RNA kit.
Tri reagent and rneasy mini kit
Manufactured by Qiagen
Tri-Reagent is a phenol-based reagent used for total RNA isolation. RNeasy Mini kit is a silica-based spin column kit for purification of total RNA.
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Lab products found in correlation
2 protocols using tri reagent and rneasy mini kit
1
RNA Isolation and Quality Assessment
2
Total RNA Isolation and Quality Assessment
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Total RNA was isolated from LDM tissue using the Tri-Reagent and RNeasy Mini kit (Qiagen) with an on-column DNase treatment according to the manufacturer’s protocol. The RNA integrity was assessed on a 1% agarose gel by electrophoresis. The RNA concentration was measured by a Nano Drop ND-1000 Spectrophotometer (PEQLAB). The quality and quantity of small RNA were assessed with an Agilent 2100 Bioanalyzer (Agilent) using an Agilent small RNA kit.
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