Cc 7014

Manufactured by Lonza

The CC-7014 is a laboratory instrument designed for cell culture applications. It provides a controlled environment for the growth and maintenance of cell lines. The core function of the CC-7014 is to regulate temperature, humidity, and gas composition to support optimal cell culture conditions.

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Lab products found in correlation

Cc 2571, by Lonza (2 mentions) Hcaec, by Lonza (1 mentions) Hcasmc, by Lonza (1 mentions) Haoec, by Lonza (1 mentions) Cc 2585, by Lonza (1 mentions) Cc 2535, by Lonza (1 mentions) Haosmc, by Lonza (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Tgf β1, by R&D Systems (1 mentions)

2 protocols using cc 7014

Gene Expression Profiling of Vascular Cells

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ADAMTS7 and CHRNB4-A3-A5 mRNA levels were measured in cultured human coronary artery smooth muscle cells (HCASMC; Lonza CC-2583, Lonza Walkersville, MD), human coronary artery endothelial cells (HCAEC, Lonza CC-2585), human aortic smooth muscle cells (HAoSMC, Lonza CC-2571), human aortic endothelial cells (HAoEC, Lonza CC-2535), human aortic adventitial fibroblasts (HAoAF, Lonza CC-7014), and human acute monocytic leukemia cell line (THP-1, ATCC TIB-202). Further details are provided in eMethods.

Saleheen D., Zhao W., Young R., Nelson C.P., Ho W., Ferguson J.F., Rasheed A., Ou K., Nurnberg S.T., Bauer R.C., Goel A., Do R., Stewart A.F., Hartiala J., Zhang W., Thorleifsson G., Strawbridge R.J., Sinisalo J., Kanoni S., Sedaghat S., Marouli E., Kristiansson K., Zhao J.H., Scott R., Gauguier D., Shah S.H., Smith A.V., van Zuydam N., Cox A.J., Willenborg C., Kessler T., Zeng L., Province M.A., Ganna A., Lind L., Pedersen N.L., White C.C., Joensuu A., Kleber M.E., Hall A.S., März W., Salomaa V., O’Donnell C., Ingelsson E., Feitosa M.F., Erdmann J., Bowden D.W., Palmer C.N., Gudnason V., De Faire U., Zalloua P., Wareham N., Thompson J.R., Kuulasmaa K., Dedoussis G., Perola M., Dehghan A., Chambers J.C., Kooner J., Allayee H., Deloukas P., McPherson R., Stefansson K., Schunkert H., Kathiresan S., Farrall M., Frossard P.M., Rader D.J., Samani N.J, & Reilly M.P. (2017). Loss of Cardio-Protective Effects at the ADAMTS7 Locus Due to Gene-Smoking Interactions. Circulation, 135(24), 2336-2353.

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Transfection and TGF-β1 Stimulation of Aortic Fibroblasts and VSMCs

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Human aortic adventitial fibroblasts (AFs; CC-7014) and human VSMCs (CC-2571) were obtained from Lonza (Lonza, Walkersville, MD) and maintained in SCGM or in SmGM (Lonza, Walkersville, MD), respectively. Transfections were performed using Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA) according to the manufacturers' instructions. The transfection efficiency, monitored by the expression of a fluorescent-labeled control small interfering RNA by flow cytometry, was ≈90% for both cells types. Cells (80 000) were plated one day before transfection on 6-well plates and transfected at 40% confluence for 24 hours, followed by incubation with or without 2.5 ng/mL of TGF-β1 (R&D Systems,

Bertorello A.M., Pires N., Igreja B., Pinho M.J., Vorkapic E., Wågsäter D., Wikström J., Behrendt M., Hamsten A., Eriksson P., Soares-da-Silva P, & Brion L. (2015). Increased arterial blood pressure and vascular remodeling in mice lacking salt-inducible kinase 1 (SIK1). Circulation research, 116(4).

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