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Anti p21 ls c88585

Manufactured by LifeSpan BioSciences

The Anti-p21 #LS-C88585 is a laboratory reagent designed for research purposes. It is an antibody that targets the p21 protein, which is involved in cell cycle regulation. This product is intended for use in various research applications, such as western blotting, immunohistochemistry, and flow cytometry, where the detection and analysis of p21 may be of interest. No further details or interpretations about the intended use of this product are provided.

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2 protocols using anti p21 ls c88585

1

Immunostaining Protocol with Antigen Retrieval

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunostaining was performed with antigen retrieval (20–30′ in boiling 10mM sodium citrate pH 6.0, 0.05% Tween 20) and by incubating primary antibodies overnight at 4°C. Detection and staining was performed with Vectastain ABC kit and DAB kits (Vector laboratories). Slides were counterstained with Hematoxylin. Antibodies used: anti-APC #sc-896 and anti-AR #sc-816, from Santa Cruz Technology; anti-β-catenin #610181 (BD); anti-chromogranin A #ab1560-1, anti-MLH1 #ab92312, anti-Sox9 # ab5535, and anti-P-MEK1 ab59329 from Abcam; anti-ck17/19 #3984, anti-MSH2 #D24B5, and anti-γ-H2AX #9718, from Cell Signaling; anti-p21 #LS-C88585 (Lifespan Biosciences), and anti-p53 #CM5 (Vector Laboratories).
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2

Immunostaining Protocol with Antigen Retrieval

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunostaining was performed with antigen retrieval (20–30′ in boiling 10mM sodium citrate pH 6.0, 0.05% Tween 20) and by incubating primary antibodies overnight at 4°C. Detection and staining was performed with Vectastain ABC kit and DAB kits (Vector laboratories). Slides were counterstained with Hematoxylin. Antibodies used: anti-APC #sc-896 and anti-AR #sc-816, from Santa Cruz Technology; anti-β-catenin #610181 (BD); anti-chromogranin A #ab1560-1, anti-MLH1 #ab92312, anti-Sox9 # ab5535, and anti-P-MEK1 ab59329 from Abcam; anti-ck17/19 #3984, anti-MSH2 #D24B5, and anti-γ-H2AX #9718, from Cell Signaling; anti-p21 #LS-C88585 (Lifespan Biosciences), and anti-p53 #CM5 (Vector Laboratories).
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