Hplc cartridge

The total phenolic compounds content was determined with the Folin-Ciocalteu reagent. Gallic acid was used as a standard and the total phenolics were expressed as mg/g gallic acid equivalents (GAE) [16 ].
The finger print of T. triandra leaves extract was carried out by using gradient high performance liquid chromatography (HPLC) system. The system consists of 515 HPLC pump and 2998 Photodiode array detector of Waters company, USA. Chromatographic separation was performed using Purospher STAR, C-18 encapped (5 μm), LiChroCART 250-4.6, and HPLC-Cartridge, Sorbet Lot number HX255346 (Merk, Germany). Two mobile phases consisting of methanol and 2.5% acetic acid in deionized (DI) water were used to induce gradient elution. The injection volume was 20 μL and the flow rate was 1.0 mL/min. During HPLC analysis the solvent gradient was programmed as shown in Table 1 and data analysis was performed using Empower 3.

The fingerprint chromatogram of the developed drink was analyzed by using gradient high-performance liquid chromatography (HPLC) system. High-performance liquid chromatography (HPLC) system consisted of 515 HPLC pump and 2998 photodiode array detector (Water Company, USA). Chromatographic separation was performed using Purospher®STAR, C-18 endcapped (5 μm), LiChroCART®250-4.6, and HPLC-Cartridge, sorbet lot number HX255346 (Merk, Germany) with guard column (Merk, Germany). Methanol (A) and 7.5% acetic acid in deionized (DI) water (B) were used as mobile phases. The gradient elution was carried out at a flow rate of 1.0 ml/min with the following gradient: 0–17 min, 70%A; 18–22 min, 100%A; 23–25, 50%A; and 26–30 min, 60%A. The sample was filtered (0.45 μm, Millipore), and a direct injection of tested sample at the volume of 20 μl on the column was performed. The chromatograms were recorded at 280 nm using a UV detector and data analysis was performed using EmpowerTM3.

The phenolic profiles of mulberry extract and encapsulated mulberry extract consisting of cyanidin-3-glucoside (Sigma-Aldrich, USA), gallic acid (Sigma-Aldrich, USA), and quercetin-3-O-rutinoside (Sigma-Aldrich, USA) were determined by high-performance liquid chromatography (HPLC). Chromatography was performed by using a Waters® system equipped with a Waters 2998 photodiode array detector. Chromatographic separation was performed using Purospher® STAR, C-18 encapped (5 μm), LiChroCART® 250-4.6, and HPLC-Cartridge, Sorbet Lot number HX255346 (Merk, Germany). The mobile phase (HPLC grade) consisted of 100% methanol (solvent A) (Fisher Scientific, USA) and 2.5% acetic acid (solvent B) (Fisher Scientific, USA) in deionized (DI) water was used to induce gradient elution. The gradient elution was carried out at a flow rate of 1.0 ml/min with the following gradient: 0–17 min, 70% A; 18–20 min, 100% A; and 20.5–25 min, 10% A. The sample was filtered (0.45 μm, Millipore), and a direct injection of tested sample at the volume of 20 μl on the column was performed. The chromatograms were recorded at 280 nm using UV detector, and data analysis was performed using EmpowerTM3.