Adult male crabs (Cancer borealis) were acquired from local distributors and maintained in aquaria filled with chilled (12–13°C) artificial sea water until use. Crabs were anesthetized before dissection by placing them in ice for at least 20 min. The stomatogastric nervous system including the STG, esophageal ganglion, the pair of commissural ganglia, and the motor nerves were dissected from the stomach and pinned to a saline filled, Sylgard-coated (Dow Corning) Petri dish (schematic in Fig. 1A ). The STG was desheathed, exposing the somata of the neurons for intracellular impalement. Preparations were superfused with chilled (10–13°C) physiological saline containing: 11 mm KCl, 440 mm NaCl, 13 mm CaCl2 · 2H2O, 26 mm MgCl2 · 6H2O, 11.2 mm Tris base, and 5.1 mm maleic acid with a pH of 7.4.
Petri dish
Manufactured by Dow
A Petri dish is a shallow, circular glass or plastic container used in laboratories for the cultivation and observation of microorganisms. It provides a controlled environment for growing cultures.
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Lab products found in correlation
2 protocols using petri dish
1
Crustacean Nervous System Dissection
2
Isolation and Dissection of Crab Stomatogastric Nervous System
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Adult male crabs (Cancer borealis) were acquired from local distributors and maintained in aquaria filled with chilled (12-13°C) artificial sea water until use. Crabs were anesthetized before dissection by placing them in ice for at least 20 minutes. The stomatogastric nervous system including the stomatogastric ganglion (STG), esophageal ganglion, the pair of commissural ganglia, and the motor nerves were dissected from the stomach and pinned to a saline filled, Sylgard-coated (Dow Corning) Petri dish (schematic in Figure 1A). The STG was desheathed, exposing the somata of the neurons for intracellular impalement. Preparations were superfused with chilled (10-13°C) physiological saline containing: 11 mM KCl, 440 mM NaCl, 13 mM CaCl 2 • 2H 2 O, 26 mM MgCl 2 • 6H 2 O, 11.2 mM Tris base, 5.1 mM maleic acid with a pH of 7.4.
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