10-20 μL of protein samples were incubated at the indicated temperatures and times and subsequently added into 384 well non-binding microplates (Greiner bio-one, Kremsmünster, Austria). Microscopy images were taken after droplets had settled to the bottom of the plate. Images were taken with either a confocal microscope (STORM spinning disc, Andor, Belfast, UK) with an Apo 100x/1.49 NA oil objective (Nikon Instruments, Amsterdam, Netherlands) or with a Plan Apochromat VC 60x/1.2 NA water objective (Nikon Instruments, Amsterdam, Netherlands) or widefield microscopes (DeltaVision Elite; Olympus, Tokyo, Japan and Zeiss Axiovert 200M, Jena, Germany) with an UPlan Super-Apochromat 100x 1.4 NA oil objective (Olympus, Tokyo, Japan) or with a Plan-Apochromat 40x 0.95 NA air objective (Zeiss, Jena, Germany).
Plan apochromat vc 60x 1.2 na water objective
Manufactured by Nikon
Sourced in Netherlands
The Plan Apochromat VC 60x/1.2 NA water objective is a high-quality microscope objective lens designed for water-immersion applications. It features a numerical aperture of 1.2 and a magnification of 60x, providing high-resolution imaging capabilities. The objective is designed using apochromatic correction to minimize chromatic aberrations, ensuring accurate color reproduction. The lens is also equipped with Nikon's Vibration Compensation (VC) technology, which helps to reduce the effects of external vibrations on the image quality.
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2 protocols using plan apochromat vc 60x 1.2 na water objective
1
Protein Aggregation Microscopy Imaging
2
Ola1p Microscopy Experiments with pH and Temperature
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For microscopy experiments, Ola1p and Ola1p GFP were diluted to a final concentration of 2.5 µM in a 1:10 ratio of Ola1p to Ola1p GFP in 20 mM PIPES or MES buffer of varying pH with a final salt concentration 100 mM KCl and 1 mM tris(2-carboxyethyl)phosphine (TCEP). For temperature experiments, protein samples were heated at the indicated temperature for 10 min in a thermoblock. Samples were imaged at room temperature with a Nikon Eclipse Ti2 widefield microscope and a Plan Apochromat VC 60x/1.2 NA water objective (Nikon Instruments, Amsterdam, Netherlands).
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted May 14, 2021. ; https://doi.org/10.1101/2021.05.12.443772 doi: bioRxiv preprint 29
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted May 14, 2021. ; https://doi.org/10.1101/2021.05.12.443772 doi: bioRxiv preprint 29
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