Tissues were paraffin embedded and processed following standard protocols and imaged with a Zeiss CLSM 700 confocal micro-scope. For Lung and Tumor H&E, hematoxylin (VWR, 95057–844) was stained followed by Eosin (VWR, 95057–848). The following antibodies were used for Immunohistochemistry: Rabbit anti-mouse CD8α (D4W2Z) 1:400 (Cell Signaling, 98941), anti-mouse CXCL5 1:200 (LsBio, LS-C104413), rat anti-mouse Ly6g 1:100 (Abcam, ab25377), rabbit anti-mouse β-catenin 1:100 (Cell signaling, 8480). Antigen retrieval was performed by incubation in BioCare’s Nexgen Decloaker chamber for 20 minutes at 90°C. Anti-rabbit polymers were used as secondary antibodies and Vina Green Chromogen Kit (Biocare Medical, BRR 807 AH) was used as substrate for CD8. For CXCL5, LY6G, and β-catenin, anti-rat or rodent polymers were used as secondary antibodies, and Warp Red Chromagen Kit (Biocare Medical, 901-WR806–081017) was used as substrate. ImageJ software was used for quantification.
Anti mouse cxcl5
Manufactured by LSBio
Anti-mouse CXCL5 is a laboratory reagent used to detect and quantify the presence of the CXCL5 protein in mouse biological samples. It functions as an antibody that specifically binds to the CXCL5 protein, allowing for its identification and measurement.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti mouse cd8α d4w2z, by Cell Signaling Technology (2 mentions)
Nexgen decloaker chamber, by Biocare Medical (2 mentions)
Warp red chromagen kit, by Biocare Medical (2 mentions)
Rabbit anti mouse β catenin, by Cell Signaling Technology (2 mentions)
Rat anti mouse ly6g, by Abcam (2 mentions)
Clsm 700 confocal microscope, by Zeiss (2 mentions)
Vina green chromogen kit, by Biocare Medical (2 mentions)
2 protocols using anti mouse cxcl5
1
Comprehensive Immunohistochemistry Analysis
2
Comprehensive Immunohistochemistry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissues were paraffin embedded and processed following standard protocols and imaged with a Zeiss CLSM 700 confocal micro-scope. For Lung and Tumor H&E, hematoxylin (VWR, 95057–844) was stained followed by Eosin (VWR, 95057–848). The following antibodies were used for Immunohistochemistry: Rabbit anti-mouse CD8α (D4W2Z) 1:400 (Cell Signaling, 98941), anti-mouse CXCL5 1:200 (LsBio, LS-C104413), rat anti-mouse Ly6g 1:100 (Abcam, ab25377), rabbit anti-mouse β-catenin 1:100 (Cell signaling, 8480). Antigen retrieval was performed by incubation in BioCare’s Nexgen Decloaker chamber for 20 minutes at 90°C. Anti-rabbit polymers were used as secondary antibodies and Vina Green Chromogen Kit (Biocare Medical, BRR 807 AH) was used as substrate for CD8. For CXCL5, LY6G, and β-catenin, anti-rat or rodent polymers were used as secondary antibodies, and Warp Red Chromagen Kit (Biocare Medical, 901-WR806–081017) was used as substrate. ImageJ software was used for quantification.
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