Mouse anti his monoclonal antibody

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Mouse anti-His monoclonal antibody is a laboratory reagent used to detect and purify recombinant proteins containing a His-tag. It specifically binds to the histidine (His) residues in the tag sequence, allowing for the identification and isolation of the target protein.

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Lab products found in correlation

Goat anti human igg fc hrp antibody, by Abcam (1 mentions) Goat anti mouse hrp secondary antibody, by Thermo Fisher Scientific (1 mentions) Dynabeads goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Mouse anti-His tag monoclonal antibody HRP-conjugated mouse monoclonal anti-HIS antibody Anti-His monoclonal antibody Mouse anti-His antibody Mouse anti-His Anti-His antibody Monoclonal mouse antibody

2 protocols using mouse anti his monoclonal antibody

Dissecting DPP4-RBD Interactions

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To determine the interaction between DPP4 and RBD, 1 μg of mouse anti-His monoclonal antibody (Invitrogen) and 5 μg of purified MERS-RBD-Fc-His or HKU4-RBD-Fc-His were mixed overnight at 4 °C with HEK293T cell lysates after expression of hDPP4-HA, TpDPP4-HA, and dcDPP4-HA for 48 h, respectively. The formed protein complex was then precipitated by 25 μl (1 × 10⁷) Dynabeads goat anti-mouse IgG (Invitrogen). The bounding interaction of DPP4 and RBD were analyzed by western blot. MERS-RBD-Fc-His and HKU4-RBD-Fc-His were detected by using 1:6000 dilution of goat anti-human IgG Fc (HRP) antibody (Abcam) whereas hDPP4-HA, TpDPP4-HA, and dcDPP4-HA were detected by using 1:1000 dilution of primary mouse anti-HA monoclonal antibody [HA.C5] (Abcam) and 1: 4000 dilution of secondary goat anti-mouse HRP antibody (Invitrogen).

Lau S.K., Fan R.Y., Zhu L., Li K.S., Wong A.C., Luk H.K., Wong E.Y., Lam C.S., Lo G.C., Fung J., He Z., Fok F.C., Au-Yeung R.K., Zhang L., Kok K.H., Yuen K.Y, & Woo P.C. (2021). Isolation of MERS-related coronavirus from lesser bamboo bats that uses DPP4 and infects human-DPP4-transgenic mice. Nature Communications, 12, 216.

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BDNF-AS Protein Stability Assay

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The RNH1/his,BDNF-AS plasmids or BDNF-AS siRNA were transiently transfected into MCF-7 or MCF-7R cells. CHX (100 ng/ml) was added to the DMEM culture medium, and incubation was continued for the indicated hours. The cell lysates were submitted for western blotting using mouse anti-His monoclonal antibody (Invitrogen, Carlsbad, CA, USA), and western blot data were quantified using the ImageJ software.

Lin X., Dinglin X., Cao S., Zheng S., Wu C., Chen W., Li Q., Hu Q., Zheng F., Wu Z., Lin D.C., Yao Y., Xu X., Xie Z., Liu Q., Liu Q., Yao H, & Hu H. (2020). Enhancer-Driven lncRNA BDNF-AS Induces Endocrine Resistance and Malignant Progression of Breast Cancer through the RNH1/TRIM21/mTOR Cascade. Cell reports, 31(10).

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