Bv605 anti cd8a clone 53 6.7

Manufactured by Thermo Fisher Scientific

BV605-anti-CD8a (clone 53–6.7) is a fluorochrome-conjugated monoclonal antibody that binds to the CD8a (cluster of differentiation 8a) surface antigen. CD8a is a glycoprotein expressed on the surface of cytotoxic T cells, which play a crucial role in the adaptive immune response.

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Close spelling variants of this product

Anti- CD8a (53-6.7; ), anti-CD8a (clone 53-6.7, CD8a (53-6.7; CD8a (53-6.7, Clone 53-6.7 Anti-CD8a

2 protocols using bv605 anti cd8a clone 53 6.7

Multiparameter Flow Cytometry for Immune Cell Profiling

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Staining of cells was carried out with a cocktail of primary antibodies on ice at 1:200 dilution from either BioLegend or eBioscience comprising of APC-Cy7-anti-CD45 (clone 30-F11), FITC-anti-Ly6C (clone HK1.4), PE-anti-Ly6G (clone 1A8), BV605-anti-CD11b (clone M1/70), FITC-anti-TCRγ/δ (clone GL3), PerCP/Cy5.5-anti-CD4 (clone GK1.5), and BV605-anti-CD8a (clone 53–6.7). Dead cells were stained with eFluor 450-fixable viability dye (eBioscience) at 1:1000 dilution before fixation with fixative buffer (BioLegend). The gating strategy of flow cytometry analysis is outline in Supplementary Figure 1A.

Lim C.L, & Lin V.C. (2021). Estrogen markedly reduces circulating low-density neutrophils and enhances pro-tumoral gene expression in neutrophil of tumour-bearing mice. BMC Cancer, 21, 1017.

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Multicolor Flow Cytometry Staining

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Staining of cells was carried out with a cocktail of primary antibodies on ice at 1:200 dilution from either BioLegend or eBioscience comprising of APC-Cy7-anti-CD45 (clone 30-F11), FITC-anti-Ly6C (clone HK1.4), PE-anti-Ly6G (clone 1A8), Biotin-anti-Gr1 (clone RB6-8C5), BV605-anti-CD11b (clone M1/70), FITC-anti-TCRγ/δ (clone GL3), PerCP/Cy5.5-anti-CD4 (clone GK1.5), Biotin-anti-CD3e (clone 145-2C11), and BV605anti-CD8a (clone 53-6.7). Secondary antibody staining was performed with Alexa Fluor® 647-streptavidin antibody (BioLegend). Dead cells were stained with eFluor 450-xable viability dye (eBioscience) at 1:1000 dilution before xation with xative buffer (BioLegend).

Lim C.L., & Lin V.C.L. (2021). Estrogen Markedly Reduces Circulating Low-density Neutrophils and Enhances Pro-tumoral Gene Expression in Neutrophil of Tumour-bearing Mice.

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