PBMCs were isolated from bladder cancer patients by gradient density centrifugation using Lymphoprep (Accu-Prep, 1.077g/ml, Oslo, Norway). CD11b myeloid cells were purified from PBMCs by positive selection using the anti-CD11b microbeads and columns (Miltenyi Biotec). Briefly, cells were incubated with beads conjugated with anti-mouse CD11b and positively selected on LS columns. Viability of all recovered cells was 95%, as determined by trypan blue exclusion.
Anti cd11b microbeads and columns
Manufactured by Miltenyi Biotec
Anti-CD11b microbeads and columns are laboratory instruments used for the isolation and enrichment of CD11b-positive cells from biological samples. The microbeads are coated with antibodies specific to the CD11b surface antigen, allowing for the targeted separation and purification of cells expressing this marker. The associated columns provide a platform for the efficient capture and recovery of the labeled cells.
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Lab products found in correlation
2 protocols using anti cd11b microbeads and columns
1
Isolation and Purification of CD11b+ Myeloid Cells from Bladder Cancer PBMCs
2
Isolation of CD11b+ Myeloid Cells
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PBMCs were isolated by gradient density centrifugation using Lymphoprep (Accu-Prep, 1.077g/ml, Oslo, Norway). CD11b myeloid cells were purified from PBMCs by positive selection using the anti-CD11b microbeads and columns (Miltenyi Biotec). Briefly, cells were incubated with beads conjugated with anti-mouse CD11b and positively selected on LS columns. The viability of all recovered cells was 95%, as determined by trypan blue exclusion.
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