Tissue culture reagents and media

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), propidium iodide (PI), 7-ethoxyresorufin, resorufin, α-napthoflavone, acacetin, tissue culture reagents and media, Western blotting lysis buffer and DTT were purchased from Sigma Aldrich (St Louis, MO, USA). Tangeretin and 4ʹ OH tangeretin were purchased from Apin chemicals (Abingdon, UK). Western blotting reagents were from Bio-Rad (Berkeley, CA, USA). The polyclonal antibody for CYP1A1 was from Daiichi Pure Chemicals (Gentest corporation, MA, USA), whereas the monoclonal antibodies for CYP1B1 from Auvation Limited (Glasgow, Scotland, UK), and for βactin from Cell signaling (Leiden, Netherlands). Secondary antibodies for western blotting were from Santa Cruz Biotechnology (Dallas, TX, USA).

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), propidium iodide (PI), α-napthoflavone, acacetin, apigenin, luteolin, kaempferol, quercetin, tissue culture reagents and media, Western blotting lysis buffer and DTT were purchased from Sigma Aldrich (St Louis, MO, USA). Scutellarein was purchased from Extrasynthese (Lyon, France) and 6-OH luteolin was synthesized as described in previous methodologies (Androutsopoulos et al., 2009c) . Western blotting reagents were from Bio-rad (Berkeley, CA, USA). The polyclonal antibody for CYP1B1 was from Gentest (BD Biosciences, CA, USA), whereas the monoclonal for β-actin from Cell signaling (Leiden, Netherlands). Secondary antibodies for western blotting were from Santa Cruz Biotechnology (Dallas, TX, USA).