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Sb203580

Manufactured by AG Scientific
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SB203580 is a pyridinyl imidazole compound that acts as a selective and potent inhibitor of the p38 mitogen-activated protein kinase (MAPK) pathway. It has a molecular formula of C21H16N3O and a molecular weight of 326.37 g/mol.

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Lab products found in correlation

Sp600125, by AG Scientific (3 mentions) Pd98059, by AG Scientific (3 mentions) Cobalt protoporphyrin copp, by Enzo Life Sciences (2 mentions) Ly294002, by AG Scientific (2 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions) Crocin, by Merck Group (1 mentions) Kn 92, by Cayman Chemical (1 mentions) Kn 62, by Cayman Chemical (1 mentions) Actinomycin d actd, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Tata box binding protein tbp, by Santa Cruz Biotechnology (1 mentions) Lta from staphylococcus aureus, by Merck Group (1 mentions) Actinomycin d, by Merck Group (1 mentions) Lps e coli o55 b5, by Merck Group (1 mentions) Dexamethasone (dex), by Cayman Chemical (1 mentions)

5 protocols using sb203580

1

Protoberberine Derivatives Screening

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Each of 80 protoberberine derivatives was dissolved in DMSO to a final concentration of 1 mg/ml. A stock solution containing 1 mg/ml of SP600125, SB203580, or U0126 (A.G. Scientific Inc.) was also prepared in DMSO.
Kim N., Park J., Gadhe C.G., Cho S.J., Oh Y., Kim D, & Song K. (2014). A Protoberberine Derivative HWY336 Selectively Inhibits MKK4 and MKK7 in Mammalian Cells: The Importance of Activation Loop on Selectivity. PLoS ONE, 9(4), e91037.
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2

Modulation of Myogenesis Regulators

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To suppress N-cadherin, M-cadherin, and Integrin β1, 40 nM siRNA compound (Santacruz) was incubated with Metafecten pro (Biomax) in PBS for 15 min at room temperature. Their mixtures were treated to flouting C2C12 myoblasts (1 × 105 cells/ml) and seeded on 35 mm dish. Target gene knockdown was confirmed after 48 hours.
Chemical inhibitors were used 10 mM SB203580 (A.G. Scientific) for p38MAPK inhibition, and 2 mM ethylene glycol tetraacetic acid (EGTA, Sigma) for calcium ion chelator. To confirm myogenin expression, the chemical inhibitor was removed after 4 hours because the chemical inhibitor could be harmful to myoblast survival overnight.
Youn S.W., Lee H.C., Lee S.W., Lee J., Jang H., Lee E.J, & Kim H.S. (2018). COMP-Angiopoietin-1 accelerates muscle regeneration through N-cadherin activation. Scientific Reports, 8, 12323.
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3

Investigating Crocin and LPS-Induced Cellular Responses

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Crocin (Figure 1(a)), LPS (phenol extracted from Salmonella enteritidis), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), actinomycin D (Act. D), cycloheximide (CHX), 2-(3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl)benzoic acid acetoxymethyl ester (Fluo-3/AM), and other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cobalt protoporphyrin (CoPP) and zinc protoporphyrin IX (ZnPP) were bought from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). LY294002, PD98059, SP600125, and SB203580 were obtained from A.G. Scientific (San Diego, CA, USA). Nifedipine, 1,2-bis (o-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA/AM), and calmidazolium chloride were acquired from Calbiochem (EMD Millipore, Billerica, MA, USA). KN92, KN93, and KN62 were purchased from Cayman Chemical (Ann Arbor, MI, USA) and Calbiochem.
Kim J.H., Park G.Y., Bang S.Y., Park S.Y., Bae S.K, & Kim Y. (2014). Crocin Suppresses LPS-Stimulated Expression of Inducible Nitric Oxide Synthase by Upregulation of Heme Oxygenase-1 via Calcium/Calmodulin-Dependent Protein Kinase 4. Mediators of Inflammation, 2014, 728709.
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4

Anti-inflammatory Effects of Dioscorea moniliforme Extract

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Cobalt protoporphyrin (CoPP) was purchased from Enzo Life Sciences, Inc. (USA). Actinomycin D (AD), cycloheximide (CHX), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and LTA from Staphylococcus aureus were purchased from Sigma-Aldrich Co. (USA). LY294002, PD98059, SP600125 and SB203580 were obtained from A.G. Scientific (USA). The antibodies for iNOS, HO-1, Nrf2, Akt, p38, TATA-box binding protein (TBP) and histone deacetylases (HDAC) were purchased from Santa Cruz Biotechnology (USA), the antibodies for phospho-Akt and phospho-p-38 were purchased from Cell Signaling Technology (USA), and the anti-tubulin antibody was purchased from Bio Genex (USA). The dry shoot systems of D. moniliforme Sw. were obtained and extracted as described previously [30] . In brief, the dry shoot systems (300 g) were extracted with distilled water at 100ºC for 4 h. The extract was filtered through a 0.45-μm filter, freeze-dried (yield: 8 g), and stored at 4ºC. The dried extract was dissolved in phosphate-buffered saline (PBS) and filtered through a 0.22 μm-filter before use.
Lee Y.J., Kim J.H, & Kim Y. (2018). Dendrobium moniliforme Stem Extract Inhibits Lipoteichoic Acid-Induced Inflammatory Responses by Upregulation of Heme Oxygenase-1. Journal of microbiology and biotechnology, 28(8).
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5

Immune Modulation by SARS-CoV-2 Proteins

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GMI (Cat: 767593), dissolved in sterilized PBS (1 mg/mL), was obtained from MycoMagic Biotechnology Co., Ltd. (New Taipei, Taiwan) [22] . LPS (E. coli O55:B5) was purchased from Sigma-Aldrich. SARS-CoV-2-envelope (E) protein (NBP2–90986) was purchased from Novus Biologicals (CO, USA). SARS-CoV-2-spike (S) protein (SPN-C52H9) was purchased from ACROBiosystems (DE, USA). Phorbol 12-myristate 13-acetate (PMA; P-1039-1MG) was purchased from AG Scientific (CA, USA). Dexamethasone was purchased from Cayman Chemical (Ann Arbor, MI, USA). PD98059 (ERK inhibitor; 167869-21-8) and SB203580 (P38 inhibitor; 152121-47-6) were purchased from AG Scientific (CA, USA).
Lin Z.H., Yeh H., Lo H.C., Hua W.J., Ni M.Y., Wang L.K., Chang T.T., Yang M.H, & Lin T.Y. (2023). GMI, a fungal immunomodulatory protein, ameliorates SARS-CoV-2 envelope protein-induced inflammation in macrophages via inhibition of MAPK pathway. International Journal of Biological Macromolecules, 241, 124648.
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