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Lipid stocks of 1 3 bis 1 2 dimyristoyl sn glycero 3 phospho sn glycerol

Manufactured by Avanti Polar Lipids
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Sourced in United States

Lipid stocks of 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol are synthetic lipid compounds consisting of a glycerol backbone with two myristoyl fatty acid chains and two phosphoglycerol moieties. This product is intended for use in various biochemical and biophysical research applications.

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Lab products found in correlation

Cyclosporin a csa, by Merck Group (2 mentions)

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Lipid stocks (4 citations)

2 protocols using lipid stocks of 1 3 bis 1 2 dimyristoyl sn glycero 3 phospho sn glycerol

1

Cardiolipin and Cyclosporin A Binding to P-gp

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Lipid stocks of 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol (Avanti Polar Lipids Inc., Alabama USA) (CDL 14:0) were prepared at a concentration of 10 mg/mL and diluted 50 fold in 200 mM ammonium acetate containing 2×CMC DDM. Lipid was mixed with P-gp in an appropriate ratio to observe multiply bound species. Binding of cyclosporin A (CsA) (Sigma Aldrich) and concomitant binding was performed in a similar fashion. Spectra of P-gp bound to cardiolipin 14:0, bound to cyclosporin A and to both cardiolipin and cyclosporin were acquired at an increased transient time of 128 ms (35,000 resolution at m/z 200).
Gault J., Donlan J.A., Liko I., Hopper J.T., Gupta K., Housden N.G., Struwe W.B., Marty M.T., Mize T., Bechara C., Zhu Y., Wu B., Kleanthous C., Belov M., Damoc E., Makarov A, & Robinson C.V. (2016). High-resolution mass spectrometry of small molecules bound to membrane proteins. Nature methods, 13(4), 333-336.
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2

Cardiolipin and Cyclosporin A Binding to P-gp

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lipid stocks of 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol (Avanti Polar Lipids Inc., Alabama USA) (CDL 14:0) were prepared at a concentration of 10 mg/mL and diluted 50 fold in 200 mM ammonium acetate containing 2×CMC DDM. Lipid was mixed with P-gp in an appropriate ratio to observe multiply bound species. Binding of cyclosporin A (CsA) (Sigma Aldrich) and concomitant binding was performed in a similar fashion. Spectra of P-gp bound to cardiolipin 14:0, bound to cyclosporin A and to both cardiolipin and cyclosporin were acquired at an increased transient time of 128 ms (35,000 resolution at m/z 200).
Gault J., Donlan J.A., Liko I., Hopper J.T., Gupta K., Housden N.G., Struwe W.B., Marty M.T., Mize T., Bechara C., Zhu Y., Wu B., Kleanthous C., Belov M., Damoc E., Makarov A, & Robinson C.V. (2016). High-resolution mass spectrometry of small molecules bound to membrane proteins. Nature methods, 13(4), 333-336.
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