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C57bl 6 cd45.1 pepboyj

Manufactured by Japan SLC

The C57BL/6-CD45.1 (PepboyJ) is a laboratory mouse strain that expresses the CD45.1 allele of the Ptprc gene, which encodes the CD45 protein. This strain is widely used in research to distinguish between donor and host cells in bone marrow transplantation and other adoptive transfer experiments.

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3 protocols using c57bl 6 cd45.1 pepboyj

1

Mouse Strain Characterization for Analysis

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C57BL/6-CD45.2 and C57BL/6-CD45.1 (PepboyJ) mice were purchased from Japan SLC, Sankyo-Lab Service, or bred in-house. 6–72-week-old male mice were used for the analysis. All mice were housed in a specific pathogen-free (SPF) condition with free access to food and water. Mice were sacrificed by cervical dislocation after isoflurane anesthesia. All animal protocols were approved by the Animal Care and Use Committee of the Institute of Medical Science University of Tokyo. All efforts were made to minimize suffering.
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2

Congenic Murine Transplantation Model

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C57BL/6-CD45.2 and C57BL/6-CD45.1 (PepboyJ) mice were purchased from Japan SLC, Sankyo-Lab Service, Jackson Laboratories (000664, 002014), or bred in-house. For congenic transplantation experiments, 8–12 week-old male mice were used as donors and 8–12 week-old female mice were used as recipients. TLR2-knockout mice, TLR4-knockout mice, and NOD/Scid (NOD.Cg-Prkdcscid) mice were purchased from Jackson Laboratories (004650, 007227, 005557, and 001303, respectively). NG (NOD.Cg-Prkdcscid Il-2rγnull/SzJ) mice were purchased from In Vivo Science Inc. All mice were housed in a specific pathogen-free (SPF) condition with free access to food and water. All animal protocols were approved by the Animal Care and Use Committee of the Institute of Medical Science University of Tokyo, the Animal Care and Use Committee of RIKEN Tsukuba Branch, and/or the Administrative Panel on Laboratory Animal Care at Stanford University.
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3

Congenic Murine Transplantation Model

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C57BL/6-CD45.2 and C57BL/6-CD45.1 (PepboyJ) mice were purchased from Japan SLC, Sankyo-Lab Service, Jackson Laboratories (000664, 002014), or bred in-house. For congenic transplantation experiments, 8–12 week-old male mice were used as donors and 8–12 week-old female mice were used as recipients. TLR2-knockout mice, TLR4-knockout mice, and NOD/Scid (NOD.Cg-Prkdcscid) mice were purchased from Jackson Laboratories (004650, 007227, 005557, and 001303, respectively). NG (NOD.Cg-Prkdcscid Il-2rγnull/SzJ) mice were purchased from In Vivo Science Inc. All mice were housed in a specific pathogen-free (SPF) condition with free access to food and water. All animal protocols were approved by the Animal Care and Use Committee of the Institute of Medical Science University of Tokyo, the Animal Care and Use Committee of RIKEN Tsukuba Branch, and/or the Administrative Panel on Laboratory Animal Care at Stanford University.
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