Aldara

Manufactured by 3M

Sourced in United States, United Kingdom

Aldara is a laboratory equipment product manufactured by 3M. It is designed for use in research and scientific applications. The core function of Aldara is to provide a controlled environment for various laboratory procedures and experiments.

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Lab products found in correlation

Vaseline lanette cream, by Fagron (2 mentions) Baby lotion, by Johnson & Johnson (1 mentions) Phosphate buffered saline tablet, by Merck Group (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Amphotericin b, by Merck Group (1 mentions) Pluronic f 127, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Imiquimod, by 3M (1 mentions) Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions) Dial thickness gauge, by Mitutoyo (1 mentions) Female balb c mice, by Charles River Laboratories (1 mentions)

16 protocols using aldara

Imiquimod-Induced Skin Inflammation in Mice

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WT, TNFR1KO, TNFR2KO, and TNFR1; TNFR2-double-null mice (DKO) are in the C57BL/6 background. 8–10-week-old mice with equal number of males and females (n=6) received a daily topical dose of 80 mg (4 mg of the active compound) of commercially available imiquimod cream (Aldara, 3M Health Care Limited, UK).
The cream was applied on the shaved (using a Wahl Pocket Trimmer) dorsal skin (~4 cm² area) for 4 consecutive days as reported earlier (van der Fits et al., 2009 (link)). Mice were euthanized and skin and blood samples were collected. Control mice were treated with a baby lotion (Johnson & Johnson), which contains similar inactive ingredients as Aldara cream. The harvested skin specimens from each mouse are divided into 4 aliquots for paraffin fixation, frozen sections, RNA and protein extraction.

Chandrasekharan U.M., Kaur R., Harvey J.E., Braley C., Rai V., Lee M., de Windt N., Hsieh J., Jaini R., Bayik D., Scheraga R.G., Fernandez A.P., DiCorleto P.E, & Husni M.E. (2022). TNFR2 depletion reduces psoriatic inflammation in mice via downregulating specific dendritic cell populations in lymph nodes and inhibiting IL-23/IL-17 pathways. The Journal of investigative dermatology, 142(8), 2159-2172.e9.

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Imiquimod-Induced Psoriasis Model in Mice

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BALB/c mice (8-11 week-old) received daily topical application of 62.5mg 5% Aldara (3M Pharmaceuticals) on their shaved back skin. Control mice (n=6, pooled from two independent experiments) were treated with a thin layer of petrolatum (Fagron). Daily evaluation of the local psoriasis area and severity index (PASI) has been described previously (16 (link)). Every other day, 20 mg/kg body weight of anti-IL-10 or isotype control antibody (n=10 each, pooled from two independent experiments) was intraperitoneally (i.p.) injected, or 5 mg/kg body weight of dexamethasone (n=7, pooled from two independent experiments) was subcutaneously (s.c.) injected as an anti-inflammatory gold standard. Five and ten days after IMQ induction, mice were sacrificed for analysis. Food and water were provided ad libitum, and mice were kept under specific pathogen-free conditions. All experiments were approved by the Erasmus MC Dutch Animal Ethics Committee (DEC).

Xu X., Prens E., Florencia E., Leenen P., Boon L., Asmawidjaja P., Mus A.M, & Lubberts E. (2021). Interleukin-17A Drives IL-19 and IL-24 Expression in Skin Stromal Cells Regulating Keratinocyte Proliferation. Frontiers in Immunology, 12, 719562.

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Topical Amphotericin B Formulation Development

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Dapsone (DAP), stearic acid, cetylic alcohol, glycerol monoestearate, solid paraffin, and white vaseline were supplied by Fagron (Terrassa, Spain). Liquid paraffin was obtained from Guinama (La Pobla de Valbona, Spain). Lipoid S100^® (soybean lecithin) was kindly gifted by Lipoid GMBH (Ludwigshafen, Germany). Amphotericin B (AmB), ethylenediaminetetraacetic acid (EDTA), dimethyl sulphoxide (DMSO), sodium hydroxide, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), methanol, phosphate buffered saline tablets, and Pluronic^® F-127 were obtained from Sigma-Aldrich (St Louis, MO, USA). Miglyol 810^® and Transcutol^® were purchased by Gattefossé (Saint-Priest, France). Aldara^® (IMQ 5%) was supplied by 3M Pharmaceuticals (St. Paul, MN, USA). Acetonitrile was provided by Merck (Germany). Water (>18 MΩ/cm resistivity) was obtained from an Ultramatic Type I system (Wasserlab, Spain). All other reagents were of analytical grade and were used without further purification.

Moreno E., Calvo A., Schwartz J., Navarro-Blasco I., González-Peñas E., Sanmartín C., Irache J.M, & Espuelas S. (2019). Evaluation of Skin Permeation and Retention of Topical Dapsone in Murine Cutaneous Leishmaniasis Lesions. Pharmaceutics, 11(11), 607.

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Imiquimod-Induced Psoriasis-Like Model

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C57BL/6-TG2KO and C57BL/6-TG3KO mice were generated in our laboratory [21 (link)]. All mice used in this study were bred in the animal facility of the University of Roma “Tor Vergata” under specific pathogen-free conditions. All experiments were approved by the Institutional Animal Care and Use Committee (IACUC) and were carried out according to the Italian and European rules (D.L.116/92; C.E. 609/86; European Directive 2010/63/EU). For mice experiments licence n° 817/2016PR (Italian Ministry of Health). Male mice at 8 weeks of age were shaved using an electric shaver on the back skin and received topical applications of imiquimod (3.125 mg) from a commercially available cream (5%) daily for 10 days (Aldara; 3M Pharmaceuticals, St. Paul, MN, USA).

Piro M.C., Ventura A., Smirnov A., Saggini A., Lena A.M., Mauriello A., Bianchi L., Melino G, & Candi E. (2020). Transglutaminase 3 Reduces the Severity of Psoriasis in Imiquimod-Treated Mouse Skin. International Journal of Molecular Sciences, 21(5), 1566.

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Colitis and Psoriasis Induction Protocols

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For induction of acute colitis, 2.5 mg of TNBS¹¹ in 50% ethanol was administered to lightly anesthetized mice through a 3.5Fr catheter inserted into the rectum on day 0. The catheter tip was inserted 4 cm proximal to the anal verge, and 150 μL of fluid was slowly instilled into the colon, after which the mouse was held in a vertical position for 30 seconds. Starting at day 1 mice received an i.p. dose of vehicle (0.01% DMSO in PBS) or oligomycin (0.25 mg/kg) once a day, or ursolic acid orally (0.75 mg/kg in 1% gum acadia) twice a day. On day 2, colon tissue was collected for histology. Alternatively, colitis was induced by giving mice water containing 3% dextran sodium sulfate (DSS) (MP Biomedicals, MW=36,000–50,000) for 3 days.
For the induction of psoriasis, Aldara (a 5% cream of imiquimod; 3M Pharmaceuticals) was applied to the ear of mice daily for 5 days. Starting at day 1 mice received an i.p. dose of vehicle (0.01% DMSO in PBS) or oligomycin (0.25 mg/kg) once a day, or ursolic acid orally (0.75 mg/kg in 1% gum acadia) twice a day.

Franchi L., Monteleone I., Hao L.Y., Spahr M., Zhao W., Liu X., Demock K., Kulkarni A., Lesch C.A., Sanchez B., Carter L., Marafini I., Hu X., Mashadova O., Yuan M., Asara J.M., Singh H., Lyssiotis C.A., Monteleone G., Opipari A.W, & Glick G.D. (2017). Inhibiting Oxidative Phosphorylation in Vivo Restrains TH17 Effector Responses and Ameliorates Murine Colitis. Journal of immunology (Baltimore, Md. : 1950), 198(7), 2735-2746.

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Imiquimod-Induced Itch Modeling in Mice

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Mice were modeled after 2 days of adaptation after depilation. The mice were given a daily topical dose of 62.5 mg of a commercially available imiquimod cream (Aldara™, 3 M Pharmaceuticals, United States) on the shaved neck skin every day for 7 days. Control mice were treated similarly with a control vehicle cream (Vaseline Lanette cream; Fagron, EU). Spontaneous scratching behavior was quantified by recording 1 h of video at different time points indicated in figure legends.

Zhou G.K., Xu W.J., Lu Y., Zhou Y., Feng C.Z., Zhang J.T., Sun S.Y., Wang R.M., Liu T, & Wu B. (2023). Acid-sensing ion channel 3 is required for agmatine-induced histamine-independent itch in mice. Frontiers in Molecular Neuroscience, 16, 1086285.

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Intratumoral IMQ and Anti-CD40 Treatment

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IMQ [Aldara™ (3 M Pharmaceuticals)] was administered by intratumoral (i.t.) injection at 50 μg once daily for 6 days starting at the time of surgery. Anti-CD40 (FGK45; Ab Solutions, Perth, Australia) treatment commenced on day 19 at 100 μg administered intraperitoneally (i.p.) given every second day for three doses. For cell depletion studies, anti-CD4 (GK1.5) or anti-CD8 (YTS.169) (Ab Solutions, Perth, Australia) was administered from day 17 (1 day pre-surgery), given every second day for a total of three doses. The initial dose was given intravenously (i.v.), followed by two i.p. injections of 150 μg.

Khong A., Cleaver A.L., Fahmi Alatas M., Wylie B.C., Connor T., Fisher S.A., Broomfield S., Lesterhuis W.J., Currie A.J., Lake R.A, & Robinson B.W. (2014). The efficacy of tumor debulking surgery is improved by adjuvant immunotherapy using imiquimod and anti-CD40. BMC Cancer, 14, 969.

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Imiquimod-Induced Psoriasis Model in Mice

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Mice received five sequential daily topical doses of 62.5 mg of imiquimod cream (5%) (Aldara™; 3 M Pharmaceuticals, Maplewood, MN, USA) or Vaseline cream (Unilever, Rotterdam, Netherlands) on their shaved back, as described [28 (link)]. Mice were assessed for body weight. TEWL was measured on the back skin using a Tewameter TM 210 (Courage + Khazaka GmbH, Cologne, Germany). The composition of KSF0041 cream (with 1% KSF0041 extract) was as follows: 50 mg KSF0041 extract, 0.9 g glycerin, 3 g olive oil, and 1.2-2.4 g olive oil wax. The mixture was incubated overnight at room temperature and stored at 4 °C. A daily topical dose of 62.5 mg KSF0041 cream or vehicle cream (without KSF0041) was applied daily during the imiquimod application period. The mice were euthanized by carbon dioxide asphyxiation 5 days after induction of psoriasis-like skin inflammation.

Lim Y., Park S.H., Kim E.J., Lim H., Jang J., Hong I.S., Kim S, & Jung Y. (2023). Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice. Biological Research, 56, 40.

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Imiquimod-Induced Psoriasis Model in GILZ-Wt and GILZ-Tg Mice

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Female GILZ-Wt and GILZ-Tg mice (8–12 weeks) were used for the IMQ-induced psoriasis mouse model. Two days after shaving, Aldara^® (5% IMQ, 3 M Pharmaceuticals; 62.5 mg) or control cream (Ava, Fagron NV) was topically applied on mouse dorsal skin daily for 7 consecutive days. Macroscopic parameters such as skin erythema and scaling were daily scored independently on a scale from 0 to 4: 0, none; 1, slight; 2, moderate; 3, marked; 4, very marked. On day 7, a section of dorsal skin and gut was collected, snap-frozen and stored at −80 °C until use or fixed in 70% ethanol for histopathological analysis. Spleens were collected and weight was expressed relative to mouse body weight. The total number of animals used was 31 (15 GILZ-Wt, 16 GILZ-Tg; two independent experiments).

Carceller E., Ballegeer M., Deckers J., Riccardi C., Bruscoli S., Hochepied T., Libert C, & Pérez P. (2016). Overexpression of Glucocorticoid-induced Leucine Zipper (GILZ) increases susceptibility to Imiquimod-induced psoriasis and involves cutaneous activation of TGF-β1. Scientific Reports, 6, 38825.

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Murine Models of Atopic Dermatitis

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Mice (C57BL/6) at 6 weeks of age were shaved dorsally and treated with a topical application of 62.5 mg of imiquimod (IMQ) daily using a commercially available cream (Aldara; 3 M Pharmaceuticals) for 6–8 consecutive days. Atopic dermatitis lesions were induced in 6-week-old male BALB/c mice using dinitrochlorobenzene (DNCB). Briefly, the dorsal hair was removed, and then 100 μL of 1% (w/v) DNCB solution (dissolved in a 3:1 [v:v] mixture of olive oil and acetone) was applied to the back skin for sensitization. Five days after dorsal hair removal, 0.2% DNCB (150 μL) was used to challenge the dorsal skin three times per week for three weeks.

Kim S., Lee S.Y., Bae S., Lee J.K., Hwang K., Go H, & Lee C.W. (2020). Pellino1 promotes chronic inflammatory skin disease via keratinocyte hyperproliferation and induction of the T helper 17 response. Experimental & Molecular Medicine, 52(9), 1537-1549.

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