Human cell lines DMS-273, NCI-H446, NCI-H1304, NCI-H69, NCI-H82, NCI-H187, SW1573, A549, NCI-H226, NCI-H522, SK-MES-1 and H1299 were obtained from the American Type Culture Collection. BJ primary fibroblasts were a kind gift from prof. Reuven Agami, primary MEFs were isolated as previously described (13 (link)). All these cell lines were cultured in DMEM/F12 supplemented with 10% fetal calf serum and 100 units/ml penicillin and 100 μg/ml streptomycin or in RPMI supplemented with 10% fetal calf serum, 1 mM sodium pyruvate and 100 units/ml penicillin and 100 μg/ml streptomycin (all Gibco). LMYC5 mouse SCLC cells were previously generated from a TP53-/- RB1-/- LMYC mouse model (14 ). These cells were grown in DMEM/F12 supplemented with 10% fetal calf serum, HITES (Gibco), and penicillin/streptomycin. All cells were grown in a humidified atmosphere of 5% CO2 at 37°C.
Dms 273
Manufactured by American Type Culture Collection
Sourced in United States
The DMS-273 is a lab equipment product offered by American Type Culture Collection. It is a device designed for the cultivation and maintenance of cell cultures. The core function of the DMS-273 is to provide a controlled environment for the growth and propagation of various cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
H1299, by American Type Culture Collection (1 mentions)
Sk mes 1, by American Type Culture Collection (1 mentions)
Nci h226, by American Type Culture Collection (1 mentions)
Sw1573, by American Type Culture Collection (1 mentions)
Nci h446, by American Type Culture Collection (1 mentions)
Nci h69, by American Type Culture Collection (1 mentions)
Nci h82, by American Type Culture Collection (1 mentions)
Nci h187, by American Type Culture Collection (1 mentions)
Nci h522, by American Type Culture Collection (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Skbr3, by American Type Culture Collection (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Mda 468, by American Type Culture Collection (1 mentions)
Mda 453, by American Type Culture Collection (1 mentions)
Rrx 001, by MedChemExpress (1 mentions)
Rrx 001, by Merck Group (1 mentions)
N acetyl cysteine (nac), by Merck Group (1 mentions)
3 protocols using dms 273
1
Culturing Various Human and Mouse Cancer Cell Lines
2
Establishment and Maintenance of Cell Lines
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Human cancer cell lines SKBR3, PM1, MDA-453, MDA-468, DMS-273, PC3, FM-88, and embryonic kidney HEK293T were purchased from the American Type Culture Collection. Cells were cultured in Roswell Park Memorial Institute medium 1640 (Sigma St Louis, MO) or Dulbecco’s Modified Eagle’s medium (Sigma St Louis, MO) supplemented with 10% heat-inactivated fetal calf serum and antibiotics. Normal human mammary epithelial cells and normal lung fibroblasts were purchased from Clonetics/BioWittaker (Allendale, NJ) and maintained in complete Mammary Epithelial Cell Growth medium (Lonza, Verviers, Belgium). Human peripheral blood mononuclear cells were prepared from buffy coats obtained from normal volunteers. Monocytes were enriched from peripheral blood mononuclear cells using plastic adherence, and purification was verified by phenotypic analysis of the surface marker CD14+. Bone marrow–derived mesenchymal stem cells were prepared from bone marrow aspirates as described previously.51 (link) Approval for obtaining blood and bone marrow samples from normal volunteers was granted by Oslo University Hospital Ethics Committee.
3
Small Cell Lung Cancer Cell Culture
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Human small cell lung cancer cells (H82, SHP77, H526, H69, and DMS273) were kindly provided by Dr. Matthew Meyerson’s Laboratory at Dana-Farber Cancer Institute, USA. H82, SHP77, H526, H69 are originally purchased from the American Type Culture Collection (ATCC) and DMS273 was originally obtained from the European Collection of Cell Cultures (ECACC).
All human small cell lung cancer cells (H82, SHP77, H526, H69, and DMS273) were cultured at 37 °C in a humid atmosphere containing 5% carbon dioxide and in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS). JQ1 was purchased from Selleck Chemical (Shanghai, China), and acetylcysteine (NAC), ATRA, and RRx-001 were obtained from MedChemExpress (Shanghai, China). JQ1, ATRA, and RRx-001 were dissolved and aliquoted in DMSO (Sigma-Aldrich, Shanghai, China) and NAC was diluted in nuclease-free water.
All human small cell lung cancer cells (H82, SHP77, H526, H69, and DMS273) were cultured at 37 °C in a humid atmosphere containing 5% carbon dioxide and in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS). JQ1 was purchased from Selleck Chemical (Shanghai, China), and acetylcysteine (NAC), ATRA, and RRx-001 were obtained from MedChemExpress (Shanghai, China). JQ1, ATRA, and RRx-001 were dissolved and aliquoted in DMSO (Sigma-Aldrich, Shanghai, China) and NAC was diluted in nuclease-free water.
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