Ltep a 2

Manufactured by American Type Culture Collection

Sourced in United States

The LTEP-A-2 is a laboratory equipment product offered by American Type Culture Collection. It is designed for long-term preservation and storage of cell cultures. The core function of the LTEP-A-2 is to maintain the viability and integrity of cell samples over extended periods.

Automatically generated - may contain errors

Lab products found in correlation

19 protocols using ltep a 2

Evaluating DF Impact on Lung Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

The DF specimens were sterilized and pretreated at 0°C before co-culture assays in vitro. Sixteen DF specimens were used to evaluate the influence of DF on lung cancer cell lines. LTEP-a-2 and A549 lung cancer cell lines were acquired from American Type Culture Collection (ATCC, Manassas, VA, USA). LTEP-a-2 and A549 cells were cultured in RPMI 1640 medium (ATCC) and in F-12K medium (ATCC) supplemented with 10% FBS (Sigma-Aldrich Co., St. Louis, MO, USA). Each experiment was repeated three times.

Mao Y., Yu Y, & Han Y. (2019). Influence of thoracic drainage fluid on proliferation, migration, apoptosis, and drug resistance in lung cancer cell lines. Cancer Management and Research, 11, 2253-2259.

+ Open protocol

+ Expand

Lung Adenocarcinoma Cell Lines: Cultivation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lung adenocarcinoma cell lines, including NCI-H1650, NCI-H1299, LTEP-a 2, NCI-H1975, CaLu-3, A549, PG49, NCI-H358, NCI-H1299 and HEY-293T, were obtained from ATCC. The cell lines were maintained in Roswell Park Memorial Institute (RPMI) -1640 containing 10% fetal bovine serum (FBS; Invitrogen, USA).
The lung carcinoma tissues and clinical data obtained from our institute were approved by the Institutional Review Board of China (approval ID 81470137). We also subjected the cell lysates from the clinical specimens to Western blot analysis.

Zhang L., Wang Y., Zhang B., Zhang H., Zhou M., Wei M., Dong Q., Xu Y., Wang Z., Gao L., Qu Y., Shi B., Zhu J., Yin Y., Chen Y., Sun L., Zhang W., Xu S., Ying G, & Wang C. (2017). Claudin-3 expression increases the malignant potential of lung adenocarcinoma cells: role of epidermal growth factor receptor activation. Oncotarget, 8(14), 23033-23047.

+ Open protocol

+ Expand

Culturing Human Lung Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Calu-3, GLC-82 and LTEP-a-2 cell lines were from ATCC (Manassas, VA) and 95-D, A549, NCI-N460, NCI-H209 and NCI-H446 were from Shanghai Cell Bank of Chinese Academy of Science (Shanghai, China). All human lung cancer cell lines were grown in Dulbecco’s minimal essential medium or RPMI 1640 medium containing 10% fetal bovine serum and 50 units/mL penicillin and 50 μg/mL streptomycin sulfate. Cells were incubated at 37°C in a humidified atmosphere of 5% CO₂.

Xu L., Wang Z.H., Xu D., Lin G., Li D.R., Wan T, & Guo S.L. (2014). Expression of Derlin-1 and its effect on expression of autophagy marker genes under endoplasmic reticulum stress in lung cancer cells. Cancer Cell International, 14, 50.

+ Open protocol

+ Expand

X-ray Irradiation of Lung Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Four cell lines of NSCLC, including LTEP-a-2 (LTE, adenocarcinoma), and NCI-H460 (H460, large cell carcinoma), obtained from the ATCC (Manassas, VA, USA); SPC-A-1 (SPC, adenocarcinoma) and LK2 (LK, squamous carcinoma) cell lines were obtained from the Cell Bank of the Chinese Academy (Shanghai, China). Plastic flasks with lung cancer cells were treated with X-ray irradiation using a linear accelerator (Primus, Siemens, Germany) with a dose of 2Gy and 4Gy. X-ray irradiation was delivered soon after the cell density reached 70–80%, and a non-irradiated lung cancer cell line was used as control. After irradiation, the cells were harvested at the appropriate time points and stored in a freezer (− 80 °C) before being processed for further analysis.

Ma S., Zhang W.L., Leckey BD J.r., Xu H.T., Yang L.H, & Wang E. (2018). X-ray irradiation induced Disabled-2 gene promoter de-methylation enhances radiosensitivity of non-small-cell lung carcinoma cells. Journal of Experimental & Clinical Cancer Research : CR, 37, 315.

+ Open protocol

+ Expand

NSCLC Cell Lines for PVT1 Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Five common NSCLC cell lines, A549, H460, H1299, SPC-A-1, and LTEP-A-2 (ATCC, Manassas, VA, USA), were selected for this experiment. After recovery, the cell lines were cultivated in an incubator (BB15, Thermo Fisher Scientific, Waltham, MA, USA) of saturated humidity with 5% CO₂ at 37°C with DMEM (No. 12800017, GIBCO, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS) (No. 26140079, GIBCO, Grand Island, NY, USA). Cell passage was conducted when the cell confluence reached 90%. The culture solution was removed and the cells were washed twice with PBS. Until the cells were isolated into single-cell and presented in round shape after the detachment with 0.25% trypsin, DMEM containing 10% FBS was added to terminate digestion. A transfer liquid gun was employed to percuss the cells to single-cell suspension. The expression of PVT1 in these five cell lines was detected by qRT-PCR, and the highest two were to be used in later experiments.

Wang D, & Hu Y. (2018). Long Non-coding RNA PVT1 Competitively Binds MicroRNA-424-5p to Regulate CARM1 in Radiosensitivity of Non-Small-Cell Lung Cancer. Molecular Therapy. Nucleic Acids, 16, 130-140.

+ Open protocol

+ Expand

Transfection of Human Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human bronchial epithelial cells (HBEs), a human adenocarcinoma cell line (A549, LTEP-a-2, H1299), and an SCC cell line (LK-2, SK-MES-1) were purchased from ATCC (Manassas, VA, USA). The SIPA1L3-siRNA and the negative controls were purchased from Genechem Biological Technology Co. Ltd. (Shanghai, China). The cells were transfected with plasmids using Attractene transfection reagent (Qiagen, Hilden, Germany) according to the manufacturer's instructions.

Wang S., Han Q., Rong X., Sun M., Diao K., Wang E., & Liu Y. (2021). Knockdown of SIPA1L3 Inhibits the Proliferation and Invasion of Non–Small Cell Lung Cancer Cells Through the Hippo Pathway.

+ Open protocol

+ Expand

NSCLC Cell Lines and Culture Conditions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Six NSCLC cell lines (HTB-182, A549, SPC-A-1, H1299, PC-9, LTEP-A-2) were obtained from the American Type Culture Collection. A normal human bronchial epithelial cell line (HBE), were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in RPMI 1640 (GIBCO-BRL) medium supplemented with 10 % fetal bovine serum (10 % FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin (Biyuntian, China) in humidified air at 37 °C with 5 % CO2.

Chen W., Wang J., Liu S., Wang S., Cheng Y., Zhou W., Duan C, & Zhang C. (2016). MicroRNA-361-3p suppresses tumor cell proliferation and metastasis by directly targeting SH2B1 in NSCLC. Journal of Experimental & Clinical Cancer Research : CR, 35, 76.

+ Open protocol

+ Expand

Cell Culture Protocols for NSCLC and Renal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human non-small cell lung cancer cell lines used in this study were H-125, H1299, LTEP-A-2, SPC-A-1, and NCL-H446, which were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). Human renal epithelial 293T cells were purchased from the Cell Bank Type Culture Collection of the Chinese Academy of Sciences (CBTCCCAS, Shanghai, China). All cells were grown in DMEM (GIBCO, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS; GIBCO) at 37°C in a humidified atmosphere of 5% CO 2 .

Chen G.M., Ding R.F., Tan Y.D., Pan X.B., Jiang G.M., He J.F., Lin S.H., Liu C, & Jia Y. (2015). Role of the CKIP1 gene in proliferation and apoptosis of the human lung cancer cell line H1299. Genetics and molecular research : GMR, 14(2).

+ Open protocol

+ Expand

Culturing Human Bronchial Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human bronchial epithelial cells (HBE), NSCLC cell lines H292, H460 and LTEP-A-2 were obtained from American Type Culture Collection (ATCC; Manassas, VA, U.S.A.). Cells were cultured in a medium containing 10% fetal bovine serum (FBS; HyClone Laboratories Inc., Logan, UT, U.S.A.) and 1% penicillin–streptomycin (Invitrogen, Carlsbad, CA, U.S.A.) at 37°C in the presence of 5% CO₂. Media containing cells were passaged every 2–3 days. Four independent series of treatments were conducted to obtain the three technical repeats that were used for all studies.

Zhu M., Zhang H., Lu F., Wang Z., Wu Y., Chen H., Fan X., Yin Z, & Liang F. (2021). USP52 inhibits cell proliferation by stabilizing PTEN protein in non-small cell lung cancer. Bioscience Reports, 41(10), BSR20210486.

+ Open protocol

+ Expand

Culturing Human Lung Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human LUAD cell lines A549, Calu-3, and LTEP-a-2 (American Type Culture Collection; ATCC, USA) as well as normal control cell BEAS2B (ATCC, USA) were routinely cultured in RPMI-1640 medium supplement with 10% FBS, 100 U/mL penicillin, and 0.1 mg/mL streptomycin at 37℃ with 5% CO₂.

Wang J., Xu M., Li D.D., Abudukelimu W, & Zhou X.H. (2020). GPR37 promotes the malignancy of lung adenocarcinoma via TGF-β/Smad pathway. Open Medicine, 16(1), 24-32.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!