A PC-10 vertical Micropipette Puller (Narishige International Inc., East Meadow, NY) was used to make recording electrodes with resistances of 3–8 MΩ from borosilicate glass pipettes (GC150F-10, Harvard Apparatus, Holliston, MA). Recordings were obtained using an Axopatch 200B amplifier (Molecular Devices, Sunnyvale, CA), where both membrane capacitance and series resistance were estimated using the dial settings on the amplifier, and both capacitive transients and series resistances were compensated by 70–80%. Data acquisition and filtering occurred at 20 kHz and 5 kHz, respectively, before digitization and storage. Clampex 9 software (Molecular Devices) was used to set experimental parameters, and electrophysiological equipment was interfaced to this software using a Digidata 1200 analog–digital interface (Molecular Devices).
Pc 10 vertical micropipette puller
Manufactured by Narishige
Sourced in Germany
The PC-10 is a vertical micropipette puller manufactured by Narishige. It is a device used for the fabrication of micropipettes and microelectrodes by applying heat and mechanical force to pull and shape glass or quartz tubing into the desired fine-tipped form.
Automatically generated - may contain errors
2 protocols using pc 10 vertical micropipette puller
1
Borosilicate Glass Micropipette Fabrication
2
Extracellular Recording of CA3 LFPs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The local field potential (LFP) was recorded with glass electrodes (resistance of 1-2 MOhm) made from GB150F-8P borosilicate filaments (Science Products GmbH, Hofheim, Germany) with a PC-10 vertical micropipette puller (Narishige International, London, United Kingdom) and back-filled with recording solution. The electrode was positioned in stratum pyramidale of the CA3 region with a mechanical micromanipulator (MM 33; M€ arzh€ auser, Wetzlar, Germany). LFPs were recorded with an EXT 10-2F amplifier in an EPMS-07 housing (npi Electronic GmbH, Tamm, Germany), low-pass filtered at 3 kHz, and digitized at 5 kHz or 10 kHz with a CED 1401 interface in Spike2 software (Cambridge Electronic Design, Cambridge, United Kingdom) for offline analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!