F4 80 hybridoma cell line

Manufactured by American Type Culture Collection

Sourced in United States

The F4/80 hybridoma cell line is a well-established mouse macrophage-specific cell line. It can be used to produce the F4/80 monoclonal antibody, which is a widely used marker for the identification and isolation of mouse macrophages.

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Lab products found in correlation

Cell culture media, by Thermo Fisher Scientific (2 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Hrp conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)

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Hybridoma cell lines (4 citations)

2 protocols using f4 80 hybridoma cell line

Immune Cell Marker Expression Analysis

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All chemicals were purchased from Sigma-Aldrich (St. Louis, MO) and cell culture media was purchased from Invitrogen (Carlsbad, CA) unless specially indicated. The F4/80 hybridoma cell line is from American Tissue Culture Collection (ATCC, Manassas, VA, USA). Primary antibodies included in the study are listed in Table 1. Alexa Fluor-conjugated secondary antibodies were purchased from Invitrogen.Table 1

Primary antibodies

Epitope	Catalog Number	Manufacturer
ABCA1	89352-256	Genetex
ABCG1	sc-650	Santa Cruz
Arginase-1	4685	Cell Signaling
GAPDH	2118	Cell Signaling
GFAP	Z0334	Dako
Gr-1	RB6-8C5	eBioscience

Guo L., Rolfe A.J., Wang X., Tai W., Cheng Z., Cao K., Chen X., Xu Y., Sun D., Li J., He X., Young W., Fan J, & Ren Y. (2016). Rescuing macrophage normal function in spinal cord injury with embryonic stem cell conditioned media. Molecular Brain, 9, 48.

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Immunohistochemical Staining Protocols

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All the chemicals were purchased from Sigma (St. Louis, MO, USA) and cell culture media were purchased from Invitrogen (Carlsbad, CA, USA) unless specifically noted. The F4/80 hybridoma cell line was from American Tissue Culture Collection (ATCC, Manassas, VA, USA). Recombinant mouse IL-34 was from R&D Systems (Minneapolis, MN, USA). The primary antibodies used in the study are listed in Table 1. All Alexa Fluor- or HRP-conjugated secondary antibodies were from Invitrogen.

Chen T., Wang X., Guo L., Wu M., Duan Z., Lv J., Tai W., Renganathan H., Didier R., Li J., Sun D., Chen X., He X., Fan J., Young W, & Ren Y. (2014). Embryonic Stem Cells Promoting Macrophage Survival and Function are Crucial for Teratoma Development. Frontiers in Immunology, 5, 275.

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