Authentic (−)-deguelin, rapamycin for in vitro studies and enzalutamide/MDV3100 were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). rapamycin for animal studies was purchased from LC Laboratories (Woburn, MA, USA). R1881 was purchased from Perkin Elmer (Waltham, MA, USA). Sulforhodamine B salt, paclitaxel, 17-AAG, crystal violet, Trizma, Dulbecco’s phosphate-buffered saline (DPBS), HEPES, hydrocortisone, insulin, phenylmethanesulfonyl fluoride (PMSF), carboxymethyl cellulose, Tween-80, polyethylene glycol 400 and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Compounds for cell treatments were dissolved in DMSO and stored at −20°C. Compounds for in vivo studies were stored as stock solutions in DMSO (enzalutamide) or ethanol (rapamycin) at −20°C and diluted immediately before use.
Enzalutamide mdv3100
Manufactured by Cayman Chemical
Sourced in United States
Enzalutamide/MDV3100 is a synthetic small molecule that functions as an androgen receptor antagonist. It is designed to inhibit the activity of the androgen receptor, which is a protein involved in the development and progression of certain types of prostate cancer.
Automatically generated - may contain errors
Lab products found in correlation
Carboxymethylcellulose, by Merck Group (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Hepes, by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
Polyethylene glycol 400, by Merck Group (1 mentions)
Dulbecco s phosphate buffered saline dpbs, by Merck Group (1 mentions)
17 aag, by Merck Group (1 mentions)
Phenylmethanesulfonyl fluoride pmsf, by Merck Group (1 mentions)
Rapamycin, by Cayman Chemical (1 mentions)
Trizma, by Merck Group (1 mentions)
R1881, by PerkinElmer (1 mentions)
Sulforhodamine b salt, by Merck Group (1 mentions)
Rapamycin, by LC Laboratories (1 mentions)
Fc block, by BD (1 mentions)
2 protocols using enzalutamide mdv3100
1
Acquisition and Preparation of Compounds for Cell and Animal Studies
2
Antibody Staining and Cytokine Treatment
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Antibodies used include 1 : 1000 diluted EP4 (C-4) mouse antibody (Santa Cruz Biotechnology, Santa Barbara, CA), 1 : 1000 diluted neuron specific enolase (A-5) mouse antibody (NSE, Santa Cruz Biotechnology, Santa Barbara, CA), 1 : 500 diluted synaptophysin (H-8) mouse antibody (SYP, Santa Cruz Biotechnology, Santa Barbara, CA), 1 : 5000 GAPDH rabbit antibody (Sigma, Oakville, ON), 1 : 5 diluted PDL1 (MIH1) mouse antibody (BD Pharmingen, Canada), and 25 µg/mL FC block (BD Pharmingen, Canada). Human IL10 and IL6 were from StemCell Technologies (Vancouver, Canada). Enzalutamide (MDV 3100) was from Cayman Chemical Company (Ann Arbor, MI). IL10 and IL6 (StemCell Technologies) stocks were reconstituted in sterile water at 10,000 ng/mL as instructed by the manufacturer. The working solution was prepared in the growth medium and used at the indicated concentration. ENZ stock was dissolved in DMSO at 10,000 µM concentration, and the working solution was prepared in the growth medium and used at a final concentration of 10 µM.
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