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αcd4 pe

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αCD4-PE is a fluorescently-labeled monoclonal antibody that binds to the CD4 antigen, which is expressed on a subset of T lymphocytes. The PE (phycoerythrin) fluorescent dye is conjugated to the antibody, enabling detection and analysis of CD4-positive cells using flow cytometry.

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Lab products found in correlation

Facscanto analyzer, by BD (2 mentions) αpd 1, by Santa Cruz Biotechnology (2 mentions) Abd pe, by Santa Cruz Biotechnology (2 mentions) αb220 apc, by BioLegend (2 mentions) αcd8α pe, by Santa Cruz Biotechnology (2 mentions) αcd4 brilliant violet 421, by BD (1 mentions) α cd11b fitc, by BioLegend (1 mentions) α cd3ε 145 2c11, by BioLegend (1 mentions) Anti il 2, by R&D Systems (1 mentions) Anti ly6g pe, by BD (1 mentions) α cd4 apc, by BioLegend (1 mentions) Anti ly6c percp, by Thermo Fisher Scientific (1 mentions) α cd25 pe, by BD (1 mentions) αcd11c apc, by BioLegend (1 mentions)

4 protocols using αcd4 pe

1

Immunomodulation of NOD and C57BL6 Mice

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Ten-week-old female NOD mice were randomly assigned into 4 groups and treated with one dose of αPD-1-ABD-PE (5 mg/kg), a mixture of αPD-1 (scFv, 2.5 mg/kg) and ABD-PE (2.5 mg/kg), PBS, or CP (200 mg/kg) (Santa Cruz Biotechnology) intraperitoneally. These mice were euthanized 24 hours later. Blood samples and splenocytes were collected from the mice, and red blood cells in these samples were lysed with the Ammonium-Chloride-Potassium (ACK) Lysing Buffer. The remaining cells in the samples were pelleted by a centrifugation at 300 g for 5 minutes, and stained by αB220-APC (Biolegend), αCD4-PE (Biolegend), or αCD8α-PE (Santa Cruz Biotechnology, Inc). The B220+, CD4+, and CD8+ fractions in the pellets were analyzed by flow cytometry on a BD FACSCanto Analyzer. The same experiment was conducted on 10-week-old female C57BL6 mice.
Zhao P., Wang P., Dong S., Zhou Z., Cao Y., Yagita H., He X., Zheng S.G., Fisher S.J., Fujinami R.S, & Chen M. (2019). Depletion of PD-1-positive cells ameliorates autoimmune disease. Nature biomedical engineering, 3(4), 292-305.
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2

Murine and Human Cell Analysis

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For murine cell analysis, αCD4-brilliant violet 421 (clone GK1.5, BD Biosciences, Franklin Lakes, NJ, USA), αCCR7-APC (clone: 4B12), αThy1.1-PE (clone HIS51) (both eBioscience, San Diego, CA, USA), αCD4-PE (clone: GK1.5), αCD62L-FITC (clone: MEL-14) (both Biolegend, San Diego, CA, USA), and Viability Dye (APC Cy7) (Thermo Fischer, Waltham, MA, USA) were used. Human cells were stained using Alexa Fluor 488 αCD11a/CD18 (clone m24), Alexa Fluor 647 αCD11a (clone HI111), Alexa Fluor 488 αCD197 (CCR7, clone G043H7), and APC αCD62L (clone: DREG-56) specific antibodies (all Biolegend, San Diego, CA, USA). Cells were incubated with the antibodies diluted in 50 µL of PBS containing 0.1% BSA and 0.02% NaN3 at 4°C for 30 min and analyzed by flow cytometry after one washing step. For induction of the high-affinity conformation of LFA-1, an αLFA-1 α-chain antibody (NKI-L16, 1 µg/mL, kind gift from IMJ Reinieren-Beeren and Carl Figdor, Nijmegen, Netherlands) was used.
Collenburg L., Beyersdorf N., Wiese T., Arenz C., Saied E.M., Becker-Flegler K.A., Schneider-Schaulies S, & Avota E. (2017). The Activity of the Neutral Sphingomyelinase Is Important in T Cell Recruitment and Directional Migration. Frontiers in Immunology, 8, 1007.
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3

Immunomodulation of NOD and C57BL6 Mice

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Ten-week-old female NOD mice were randomly assigned into 4 groups and treated with one dose of αPD-1-ABD-PE (5 mg/kg), a mixture of αPD-1 (scFv, 2.5 mg/kg) and ABD-PE (2.5 mg/kg), PBS, or CP (200 mg/kg) (Santa Cruz Biotechnology) intraperitoneally. These mice were euthanized 24 hours later. Blood samples and splenocytes were collected from the mice, and red blood cells in these samples were lysed with the Ammonium-Chloride-Potassium (ACK) Lysing Buffer. The remaining cells in the samples were pelleted by a centrifugation at 300 g for 5 minutes, and stained by αB220-APC (Biolegend), αCD4-PE (Biolegend), or αCD8α-PE (Santa Cruz Biotechnology, Inc). The B220+, CD4+, and CD8+ fractions in the pellets were analyzed by flow cytometry on a BD FACSCanto Analyzer. The same experiment was conducted on 10-week-old female C57BL6 mice.
Zhao P., Wang P., Dong S., Zhou Z., Cao Y., Yagita H., He X., Zheng S.G., Fisher S.J., Fujinami R.S, & Chen M. (2019). Depletion of PD-1-positive cells ameliorates autoimmune disease. Nature biomedical engineering, 3(4), 292-305.
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4

Multiparameter Flow Cytometry Protocol

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Anti-CD11b-PacBlu, αCD11b-FITC, αCD4-PE, αCD4-APC, αCD11c-APC, αCD11c-PE, αTCRvα2-PE, and αCD3ε(145-2C11) were purchased from BioLegend (San Diego, CA, USA). Anti-Ly6C-PerCP and αFoxP3-APC were products of eBioscience (San Jose, CA, USA). Anti-Ly6G-PE, αNK1.1-PE, αCD8α-PacBlu, αCD25-PE, and αCD103-Alexa Fluor 647 were products from BD Biosciences (San Jose, CA, USA). Flt3L-Fc fusion protein was purchased from BioXCell (West Lebanon, NH, USA). Anti-IL-2 was purchased from R&D Systems (Minneapolis, MN, USA). Fc-GITR-L fusion protein was produced as described previously (9 (link)).
Liao G., O’Keeffe M.S., Wang G., van Driel B., de Waal Malefyt R., Reinecker H.C., Herzog R.W, & Terhorst C. (2014). Glucocorticoid-Induced TNF Receptor Family-Related Protein Ligand is Requisite for Optimal Functioning of Regulatory CD4+ T Cells. Frontiers in Immunology, 5, 35.
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