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Apoptotic kit

Manufactured by BD
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Sourced in United States

The Apoptotic kit is a laboratory equipment designed to detect and quantify apoptosis, a form of programmed cell death, in cell samples. The kit provides the necessary reagents and protocols to perform the analysis.

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Lab products found in correlation

Cell cycle kit, by Keygen Biotech (1 mentions)

Spelling variants of this product

Apoptotic detection kit (4 citations) Annexin V-APC apoptotic detection kit (2 citations) Annexin V-FITC/PI Apoptotic Detection Kit (2 citations)

3 protocols using apoptotic kit

1

Apoptosis and Necrosis Assay in THP-1 and Macrophages

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Apoptotic kit (BD, USA) including annexin V-FITC and PI were used for analyzing the effect of JDBM extracts on apoptosis and necrosis of THP-1 and macrophages, respectively. Briefly, treated cells were harvested, washed, and suspended in Annexin-binding Buffer. After staining with Annexin-V-FITC and PI for 15min in the dark, the apoptosis or necrosis rate was measured by FACS.
Jin L., Wu J., Yuan G, & Chen T. (2018). In vitro study of the inflammatory cells response to biodegradable Mg-based alloy extract. PLoS ONE, 13(3), e0193276.
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2

Doxorubicin-induced Apoptosis in K7M2 Cells

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After K7M2 cells were cultured for seven days and collected, the cells were pooled in 12-well plates at a concentration of 3 × 105 per well. Cells were treated with DOX (50 nmol/mL) for 24 hours. Then, cell apoptosis was assayed by FCA with the apoptotic kit (BD Biosciences, USA).
He J., Chen C., Chen L., Cheng R., Sun J., Liu X., Wang L., Zhu C., Hu S., Xue Y., Lu J., Yang H., Cui W, & Shi Q. (2022). Honeycomb-Like Hydrogel Microspheres for 3D Bulk Construction of Tumor Models. Research, 2022, 9809763.
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3

Cell Cycle Analysis by Flow Cytometry

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At 48 h following transfection, at least 105 cells were collected from each group and washed twice with PBS. According to the instructions provided with the apoptotic kit (BD Biosciences), dyes were added to the cells and protected from light for 10 min, after which flow cytometry (ACEA Biosciences) was performed to analyse the cell cycle distribution with nova express software (ACEA Biosciences). Cells from the different groups were collected, with at least 105 cells for each group. The cells were then washed twice with PBS and fixed with 75% ethanol overnight, according to the instructions provided with the cell cycle kit (KeyGEN Biotech). Dyes were added sequentially, the samples were protected from light for 30 min, and flow cytometric analysis was then performed.
Tian F., Wang H., Ma H., Zhong Y, & Liao A. (2020). miR-144-3p inhibits the proliferation, migration and angiogenesis of multiple myeloma cells by targeting myocyte enhancer factor 2A. International Journal of Molecular Medicine, 46(3), 1155-1165.
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