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Pe cy5 conjugated anti mouse cd86

Manufactured by Thermo Fisher Scientific
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PE-Cy5-conjugated anti-mouse CD86 is a fluorescently-labeled monoclonal antibody that binds to the mouse CD86 cell surface protein. It can be used to detect and quantify CD86-expressing cells in flow cytometry applications.

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Lab products found in correlation

Apc conjugated anti mouse cd11c, by Thermo Fisher Scientific (1 mentions) Fitc conjugated anti mouse cd40, by Thermo Fisher Scientific (1 mentions) Pe conjugated anti mouse cd80, by Thermo Fisher Scientific (1 mentions) Fitc conjugated anti mouse cd11c, by Thermo Fisher Scientific (1 mentions) Facscan, by BD (1 mentions) Fitc annexin 5 apoptosis detection kit, by BD (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions)

2 protocols using pe cy5 conjugated anti mouse cd86

1

Multicolor Flow Cytometry Panel

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APC-conjugated anti-mouse CD11c; PE-conjugated anti-mouse MHC-II and CD11b; PE-Cy5-conjugated anti-mouse CD86; FITC-conjugated anti-mouse CD40, CD80, CD83, and GR-1, and PE-conjugated anti-mouse IFN-γ monoclonal antibodies were purchased from eBioscience (San Diego, CA, USA). Polyclonal anti-HO-1 Ab was purchased from Enzo Life Sciences (Lausen, Switzerland). Polyclonal anti-tubulin Ab was purchased from Beyotime (Shanghai, China).
Zhao Y., Jia Y., Wang L., Chen S., Huang X., Xu B., Zhao G., Xiang Y., Yang J, & Chen G. (2018). Upregulation of Heme Oxygenase-1 Endues Immature Dendritic Cells With More Potent and Durable Immunoregulatory Properties and Promotes Engraftment in a Stringent Mouse Cardiac Allotransplant Model. Frontiers in Immunology, 9, 1515.
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2

Phenotypic Analysis of Bone Marrow-Derived Dendritic Cells

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After treatment for 24 hours, BMDCs were harvested and stained with fluorescein FITC-conjugated anti-mouse CD11c, PE-conjugated anti-mouse CD11b, PE-conjugated anti-mouse CD80, PECy5-conjugated anti-mouse CD86, and PECy5-conjugated anti-mouse major histocompatibility (MHC) class II antibodies (eBioscience). The FITC Annexin V apoptosis detection kit (BD Pharmingen) was used for cell death studies. Cells were washed with PBS and fixed with 1% paraformaldehyde (Merck). Fluorescently labeled cells were analyzed by flow cytometry (FACScan, BD).
Lin I.P., Hsu Y.S., Kang S.W., Hsieh M.H, & Wang J.Y. (2014). Escherichia coli Heat-Labile Detoxified Enterotoxin Modulates Dendritic Cell Function and Attenuates Allergic Airway Inflammation. PLoS ONE, 9(3), e90293.
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