Sodium nitrite nano2

Manufactured by Sinopharm

Sourced in China

Sodium nitrite (NaNO2) is a chemical compound that is commonly used as a laboratory reagent. It is a white to slightly yellowish crystalline solid that is soluble in water. Sodium nitrite serves as a reducing agent and is often used in various analytical and synthetic applications in the laboratory setting.

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6 protocols using sodium nitrite nano2

Synthesis and Characterization of Lanthanum and Cerium Compounds

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Sodium nitrite (NaNO₂) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Sulfuric acid (H₂SO₄, 98%), H₂O₂ (35%), hydrochloric acid, and ethanol (98%) were brought from Beijing Chemical Reagent Co. (Beijing, China). Sodium fluoride (NaF, >98%), heptahydrate lanthanum chloride (LaCl₃·7H₂O, 99.9%), heptahydrate cerium chloride (CeCl₃·7H₂O, 99.9%), oleic acid (90%), 5,5-dimethyl-1-pyrroline N-oxide (DMPO), and ascorbic acid (AA) were all purchased from Sigma-Aldrich. Unless otherwise noted, all the chemicals were used without further purification.

Wang Y., Wang Y., Huang C., Chen T, & Wu J. (2020). Ultra-Weak Chemiluminescence Enhanced by Cerium-Doped LaF3 Nanoparticles: A Potential Nitrite Analysis Method. Frontiers in Chemistry, 8, 639.

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Cytotoxicity Evaluation of Chemical Reagents

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Starch, L-cysteine (Cys), glycine (Gly), urea, glucose (Glu), calcium chloride (CaCl₂), sodium chloride (NaCl), copper chloride (CuCl₂), potassium chloride (KCl), iron (III) chloride (FeCl3), glutathione (GSH), sodium sulfate (Na₂SO₄), sodium nitrate (NaNO₃), sodium sulfite (Na₂SO₃), sodium phosphate (Na₃PO₄), sodium bisulfite (NaHSO₄) and sodium nitrite (NaNO₂) were purchased from Sinopharm Chemical Reagent Company (China). Dulbecco’s modified Eagle’s medium (DMEM) medium and fetal bovine serum (FBS) were purchased from HyClone (United States). WST assay kits were purchased from Energy Chemical (China). All regents were not processed or purified prior to use.

Wang P., Zhang Y., Liu Y., Pang X., Liu P., Dong W.F., Mei Q., Qian Q., Li L, & Yan R. (2021). Starch-Based Carbon Dots for Nitrite and Sulfite Detection. Frontiers in Chemistry, 9, 782238.

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Antioxidant Capacity and Phenolic Analysis

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All reagents or solvents used were of analytical, HPLC or HPLC-MS grade. Absolute ethanol, acetone, aluminum nitrate nonahydrate (Al(NO₃)₃·9H₂O), hydrochloric acid (HCl), L-ascorbic acid, sodium hydroxide (NaOH), and sodium nitrite (NaNO₂) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, formic acid, leucine enkephalin and methanol, as well as two standards, rutin and cyanidin 3-O-glucoside, were purchased from Sigma-Aldrich (St. Louis, MO, USA). The iTaq^TM Universal SYBR^® Green Supermix was purchased from Bio-Rad Laboratories (Hercules, CA, USA).

Yu Z., Liao Y., Teixeira da Silva J.A., Yang Z, & Duan J. (2018). Differential Accumulation of Anthocyanins in Dendrobium officinale Stems with Red and Green Peels. International Journal of Molecular Sciences, 19(10), 2857.

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Phytochemical Analysis and Antioxidant Evaluation

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Standards of rutin, gallic acid, hyperoside, kaempferol-3-O-glucoside, quercetin-3-O-hexoside, luteolin-7-O-glucoside, 3-caffeoylquinic acid (3-CQA), 3,4-dicaffeoylquinic acid (3,4-diCQA), 3,5-dicaffeoylquinic acid (3,5-diCQA), 4,5-dicaffeoylquinic acid (4,5-diCQA), and 3,4,5-tricaffeoylquinic acid were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Folin–Ciocalteu reagent, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,4,6-tris (2-pyridyl)-s-triazine (TPTZ) were purchased from Sigma–Aldrich (Shanghai, China). HPLC-grade m Ethanol was purchased from Merck (Darmstadt, Germany). All other analytical-grade chemicals and reagents used in the study: Ethanol, petroleum ether, ethyl acetate, acetone, ether, chloroform, n-butanol, mEthanol, formic acid, phenol, sulfuric acid, sodium nitrite (NaNO₂), aluminum nitrate (Al(NO₃)₃·9H₂O), sodium hydroxide (NaOH), sodium carbonate, 3,5-dinitrosalicylic acid (DNS), and ferric chloride were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

Zhang C., Liu D., Wu L., Zhang J., Li X, & Wu W. (2019). Chemical Characterization and Antioxidant Properties of Ethanolic Extract and Its Fractions from Sweet Potato (Ipomoea batatas L.) Leaves. Foods, 9(1), 15.

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Chromium(VI) Removal Protocol

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All chemicals used in this work were of analytical grade. Sodium sulfide nonahydrate (Na₂S•9H₂O) was purchased from Wenjiang Chemical Technology. Iron sulfate heptahydrate (FeSO₄·7H₂O), 1, 5-diphenyl carbazide, FeS, carboxymethyl cellulose (abbreviated as CMC), Phosphoric acid, potassium permanganate(KMnO_4), urea (NH₂)₂CO, sodium nitrite(NaNO₂₎, NaCl, K₂Cr₂O₇, C₆H₁₂O₆, C₁₂H₂₂O₁₁, (C₆H₁₀O₅)n, C₂H₃O₂Na·3H₂O, CH₄N₂O, NaH₂PO_4, and Na₃PO₄ were provided from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Sulfuric acid were supplied by Xilong Science Chemical Technology. The Beef Extract, Peptone, Tryptone and yeast extract were purchased from shanghai Macklin Biochemical Co., Ltd (Shanghai, China). (NH₄)₂SO₄ and NH₄H₂PO₄ were supplied by Damao Chemical Reagent Co., Ltd (Tianjin, China). KH₂PO₄ was purchased from Hengxing Chemical Reagent Co., Ltd (Tianjin, China). Dissolve 2.0 g K₂Cr₂O₇ in 1 L of deionized water to obtain a Cr(VI) mother solution with a final concentration of 2000 mg L⁻¹.

Yu R., Man M., Yu Z., Wu X., Shen L., Liu Y., Li J., Xia M, & Zeng W. (2021). A high-efficiency Klebsiella variicola H12-CMC-FeS@biochar for chromium removal from aqueous solution. Scientific Reports, 11, 6611.

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Extracting Antioxidant Compounds from C. retusus

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Analytical grade ethanol, vitamin C (VC), and sodium nitrite (NaNO₂) were purchased from Sinopharm Chemical Reagent Co., Ltd. Rutin (>98%) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) were obtained from Aladdin Reagent Co., Ltd. (Shanghai, China). Aluminum nitrate (Al(NO₃)₃) and sodium hydroxide (NaOH) were analytical grade and provided by the Nanjing Reagent Co., Ltd., C. retusus leaves were collected on the Yuntai Mountain in Lianyungang, Jiangsu province. Purified water was obtained from Milli-Q system (Millipore, America). ethanol solution with different concentrations was prepared according to volume ratio. HPD5000 macroporous resin was obtained from Cangzhou Bon Adsorber Technology Co., Ltd.

Wang Z., Yang S., Gao Y, & Huang J. (2022). Extraction and purification of antioxidative flavonoids from Chionanthus retusa leaf. Frontiers in Bioengineering and Biotechnology, 10, 1085562.

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