Experiments were performed with male Fischer rats (body weight 230–250 g, Charles River Laboratories) and female BALB/c mice (18–22 g, Charles River Laboratories). Animals were maintained under standard diurnal conditions and were allowed access to food and water ad libitum. Animal experiments were approved by the Institutional Animal Care and Use Committee of the Université de Sherbrooke and performed in accordance with the Canadian Council on Animal Care guidelines.
Male fischer rats
Manufactured by Charles River Laboratories
Sourced in United States, Canada, Japan
Male Fischer rats are a well-established laboratory animal model used in a variety of research applications. They are a commonly used strain for studies due to their consistent characteristics and predictable responses.
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Lab products found in correlation
Female balb c mice, by Charles River Laboratories (1 mentions)
Chloroquine, by Merck Group (1 mentions)
C6628, by Merck Group (1 mentions)
Amiodarone, by Merck Group (1 mentions)
A8423, by Merck Group (1 mentions)
Matrigel, by BD (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Mem medium, by Merck Group (1 mentions)
F98 rat glioblastoma cells, by American Type Culture Collection (1 mentions)
Crf 1, by Oriental Yeast (1 mentions)
9 protocols using male fischer rats
1
Rodent Model for Drug Evaluation
2
Intracerebral Implantation of F98 Glioma Cells
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Male Fischer rats were purchased from Charles River Laboratories International Inc. (Wilmington, MA, USA). The implantation method was described previously.27 (link) F98 cells (10,000 cells in 5 µL) were prepared and implanted into the right caudate nucleus (1 mm anterior, 3 mm right of the bregma, and 6 mm deep) of the brain in 5 min. The convection-enhanced delivery (CED) procedure28 (link) was performed 10 days after implantation of F98 cells, at the same injection site using a 33 gauge Hamilton syringe. Before infusion, the burr was filled with bone wax and the needle was inserted 6.5 mm deep, retained there for 5 min and then withdrawn to 6 mm. Test articles and a control vehicle to be injected were vehicle (SH Buffer), CuSO4 (300 mM)-DSPC/Chol liposomes (copper 0.1 mg/mL, lipid 3.5 mg/mL), or Cu(DDC)2 formulated in DSPC/Chol liposomes (0.5 mg/mL, lipid 3.5 mg/mL). A total volume of 10 µL was infused at an infusion rate of 0.5 µL/min for 20 min (5 µg Cu(DDC)2 per rat). After the infusion, the needle was left in, to reduce backflow and increase convection volume, for 5 min prior to being withdrawn.
3
Hepatocarcinogenesis Induction and Modulation
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Male Fischer rats were obtained from Charles River (Milano, Italy). Guidelines for Care and Use of Laboratory Animals were followed during the investigation. All animal procedures were approved by the Ethical Commission of the University of Cagliari and the Italian Ministry of Health. Animals were treated with a single dose of diethylnitrosamine (DENA, 150 mg/kg) and, two weeks later, were subjected to the R-H protocol, consisting of a 2 week-diet supplemented with 0.02% 2-acetylaminofluorene (2-AAF) and a two/third partial hepatectomy (PH) [19 (link)]. Rats were then switched to basal diet all throughout the experiment and sacrificed 10 weeks after DENA administration (See Supp. Figure 1A .)
Another group of rats exposed to R-H protocol was given four doses of Chloroquine (50 mg/kg, Sigma-Aldrich, C6628) or Amiodarone (30 mg/kg, Sigma-Aldrich, A8423) starting 2 weeks after 2-AAF withdrawal (6 weeks after treatment with DENA). Rats were sacrificed 7 days after the first dose (SeeSupp. Figure 1B and 1C ). BrdU was given in drinking water (1mg/1ml) for 5 days before the sacrifice.
Another group of rats exposed to R-H protocol was given four doses of Chloroquine (50 mg/kg, Sigma-Aldrich, C6628) or Amiodarone (30 mg/kg, Sigma-Aldrich, A8423) starting 2 weeks after 2-AAF withdrawal (6 weeks after treatment with DENA). Rats were sacrificed 7 days after the first dose (See
4
Establishment of Rat Glioma Xenograft Model
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Male Fischer rats from Charles River Japan, Inc. were housed under a 12-h light/12-h dark cycle and had free access to food and water. A rat glioma C6 cell line, which was derived from gliomas induced by N-nitrosomethylurea, was provided by the RIKEN BRC. The cells were cultured in MEM medium (Sigma-Aldrich) with 10% fetal bovine serum (Sigma-Aldrich) at 37 °C in a humidified incubator containing 5% CO2. Cultured cells were collected after washing in PBS and harvested with trypsin. Tumor xenograft models were established by the subcutaneous injection of 1 × 10^6 tumor cells suspended in 0.2 mL of culture medium and Matrigel (1:1; BD Biosciences) into the bilateral shoulder region of F344 rats (a total of two sites in the left and right shoulder per one rat). PET experiments were performed 2 to 3 weeks after the implantation of C6 glioma cells, when the tumor diameter had reached 1–2 cm.
5
Comparative Aging Study on Fischer Rats
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Male Fischer rats were purchased from Charles River Laboratories International. Groups of nine young-adult rats (10 months old) and nine older rats (22 months old) were compared. The Georgetown University Animal Care and Use Committee approved all animal experiments (ethical protocol code: 2017-0056), and the investigation conformed to the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals.
6
Liver Regeneration in Rat Carcinogenesis Model
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Male Fischer rats were obtained from Charles River, Milano, Italy. Guidelines for Care and Use of Laboratory Animals were followed during the investigation. All animal procedures were approved by the Ethical Commission of the University of Cagliari and the Italian Ministry of Health. Animals were treated with a single dose of diethylnitrosamine (DENA, 150 mg/kg, Sigma-Aldrich, Milano, Italy) and, two weeks later, were subjected to the R-H protocol, consisting of a 2-week diet supplemented with 0.02% 2-acetylaminofluorene (2-AAF) and a two/thirds partial hepatectomy (PH) [27 (link)]. Rats were sacrificed 3 or 7 days after PH or switched to basal diet all throughout the experiment and sacrificed 5 and 10 weeks after DENA administration (Supp. Figure 1A ).
7
Glioblastoma in Fischer Rats
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F98 rat glioblastoma cells were purchased from American Type Culture Collection. Male Fischer rats of 210–225 g were purchased from Charles River Laboratories (Saint-Constant, Canada). The experimental animal protocol was approved by the institutional ethical committee and complied with the regulations of the Canadian Council on Animal Care (protocol # 329-13B).
8
Rat Liver Carcinogenesis Model
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Guidelines for Care and Use of Laboratory Animals were followed during the investigation. All animal procedures were approved by the Ethical Commission of the University of Cagliari and the Italian Ministry of Health. Male Fischer rats (Charles River, Milano, Italy) were treated with a single dose of DENA (150 mg/kg) and, two weeks later, subjected to the RH protocol, consisting of a 2 weeks-diet supplemented with 0.02% 2-AAF and a 2/3 PH [23 ]. A group of animals was sacrificed 7 days after PH, while the remaining rats were then switched to basal diet and sacrificed ten weeks or fourteen months after DENA administration.
9
Acclimating Male Fischer Rats for Experiments
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Male Fischer rats at 8 weeks of age were purchased from Charles River Japan, Inc. (Kanagawa, Japan). Animals were housed in stainless steel wire bracket cages in a room that was maintained at a temperature of 21 to 26°C with a relative humidity of 30 to 70%, and lighting was maintained on a 12-hr light (7:00 to 19:00)/day cycle. All animals were allowed free access to water and a commercial rodent diet (CRF-1, Oriental Yeast Co., Ltd.). Rats were acclimatized for 1 week before use. All experimental procedures were performed in accordance with the guidelines of the Animal Care and Use Committee of Daiichi Sankyo Co., Ltd (Tokyo, Japan). The experimental protocols were approved by the Ethics Review Committee for Animal Experimentation of Daiichi Sankyo Co., Ltd.
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